ArticleBMC molecular biology2010
New miRNA labeling method for bead-based quantification.
Article in BMC molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Validation of a Bead-Based Multiplex Assay for miRNA Quantification in Rat Liver Inflammation.ACS omega · 2026Article
- MicroRNA-195a-5p Regulates Blood Pressure by Inhibiting NKCC2A.Hypertension (Dallas, Tex. : 1979) · 2023Article
- Urinary Biomarkers for the Noninvasive Detection of Gastric Cancer.Journal of gastric cancer · 2022Review
- Single-Cell RNAseq Analysis of lncRNAs.Methods in molecular biology (Clifton, N.J.) · 2021Article
- High sensitivity and specificity of a 5-analyte protein and microRNA biosignature for identification of active tuberculosis.Clinical & translational immunology · 2021Article
- Epigenetics for Clinicians from the Perspective of Pediatric Rheumatic Diseases.Current rheumatology reports · 2020Review
- A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs.International journal of molecular sciences · 2020Review
- Urinary microRNA in kidney disease: utility and roles.American journal of physiology. Renal physiology · 2019Review
- A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads.Scientific reports · 2019Article
- A Uniform System for the Annotation of Vertebrate microRNA Genes and the Evolution of the Human microRNAome.Annual review of genetics · 2015Review
- MicroRNA-27a Contributes to Rhabdomyosarcoma Cell Proliferation by Suppressing RARA and RXRA.PloS one · 2015Article
- MicroRNA-27a directly targetsOncology letters · 2015Article
- Article
- Linkage of microRNA and proteome-based profiling data sets: a perspective for the priorization of candidate biomarkers in renal cell carcinoma?Journal of proteome research · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundmicroRNAs (miRNAs) are small single-stranded non-coding RNAs that act as crucial regulators of gene expression. Different methods have been developed for miRNA expression profiling in order to better understand gene regulation in normal and pathological conditions. miRNAs expression values obtained from large scale methodologies such as microarrays still need a validation step with alternative technologies.
resultsHere we have applied with an innovative approach, the Luminex xMAP technology validate expression data of differentially expressed miRNAs obtained from high throughput arrays. We have developed a novel labeling system of small RNA molecules (below 200 nt), optimizing the sensitive cloning method for miRNAs, termed miRNA amplification profiling (mRAP). The Luminex expression patterns of three miRNAs (miR-23a, miR-27a and miR-199a) in seven different cell lines have been validated by TaqMan miRNA assay. In all cases, bead-based meas were confirmed by the data obtained by TaqMan and microarray technologies.
conclusionsWe demonstrate that the measure of individual miRNA by the bead-based method is feasible, high speed, sensitive and low cost. The Luminex xMAP technology also provides flexibility, since the central reaction can be scaled up with additional miRNA capturing beads, allowing validation of many differentially expressed miRNAs obtained from microarrays in a single experiment. We propose this technology as an alternative method to qRT-PCR for validating miRNAs expression data obtained with high-throughput technologies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.