ArticleMolecular microbiology2010
Polyphosphate binds to the principal sigma factor of RNA polymerase during starvation response in Helicobacter pylori.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- The Protein Scaffolding Functions of Polyphosphate.Journal of molecular biology · 2024Review
- Polyphosphate kinase regulates LPS structure and polymyxin resistance during starvation in E. coli.PLoS biology · 2024Article
- Article
- Polyphosphate kinase 1 is involved in formation, the morphology and ultramicrostructure of biofilm ofHeliyon · 2023Article
- Article
- Transformation ofPathogens (Basel, Switzerland) · 2020Review
- Inorganic polyphosphate accumulation suppresses the dormancy response and virulence inThe Journal of biological chemistry · 2019Article
- Overexpression of spoT gene in coccoid forms of clinical Helicobacter pylori isolates.Folia microbiologica · 2018Article
- Polyphosphate Kinase Antagonizes Virulence Gene Expression in Francisella tularensis.Journal of bacteriology · 2018Article
- Transcriptome analysis of Campylobacter jejuni polyphosphate kinase (ppk1 and ppk2) mutants.Virulence · 2015Article
- Positively-charged semi-tunnel is a structural and surface characteristic of polyphosphate-binding proteins: an in-silico study.PloS one · 2015Article
- Molecular complementarity between simple, universal molecules and ions limited phenotype space in the precursors of cells.Biology direct · 2014Article
- Helicobacter pylori RNA polymerase α-subunit C-terminal domain shows features unique to ɛ-proteobacteria and binds NikR/DNA complexes.Protein science : a publication of the Protein Society · 2014Article
- Polyphosphate deficiency in Mycobacterium tuberculosis is associated with enhanced drug susceptibility and impaired growth in guinea pigs.Journal of bacteriology · 2013Article
- Fluorometric quantification of polyphosphate in environmental plankton samples: extraction protocols, matrix effects, and nucleic acid interference.Applied and environmental microbiology · 2013Article
- Article
- The Bordetella pertussis model of exquisite gene control by the global transcription factor BvgA.Microbiology (Reading, England) · 2012Review
- Growth rate regulation in Escherichia coli.FEMS microbiology reviews · 2012Review
- Polyphosphate deficiency affects the sliding motility and biofilm formation of Mycobacterium smegmatis.Current microbiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Helicobacter pylori persists deep in the human gastric mucus layer in a harsh, nutrient-poor environment. Survival under these conditions depends on the ability of this human pathogen to invoke starvation/stress responses when needed. Unlike many bacteria, H. pylori lacks starvation/stress-responding alternative sigma factors, suggesting an additional mechanism might have evolved in this bacterium. Helicobacter pylori produces polyphosphate; however, the role and target of polyphosphate during starvation/stress have not been identified. Here we show that polyphosphate accumulated during nutrient starvation directly targets transcriptional machinery by binding to the principal sigma factor in H. pylori, uncovering a novel mechanism in microbial stress response. A positively charged Lys-rich region at the N-terminal domain of the major sigma factor is identified as the binding region for polyphosphate (region P) in vivo and in vitro, revealing a new element in sigma 70 family proteins. This interaction is biologically significant because mutant strains defective in the interaction undergo premature cell death during starvation. We suggested that polyphosphate is a second messenger employed by H. pylori to mediate gene expression during starvation/stress. The putative 'region P' is present in sigma factors of other human pathogens, suggesting that the uncovered interaction might be a general strategy employed by other pathogens.
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Registered trials
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