ArticleMethods in molecular biology (Clifton, N.J.)2010
Physiological studies of the interaction between opsin and chromophore in rod and cone visual pigments.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Mechanisms of vitamin A metabolism and deficiency in the mammalian and fly visual system.Developmental biology · 2021Review
- Sensory Transduction in Photoreceptors and Olfactory Sensory Neurons: Common Features and Distinct Characteristics.Frontiers in cellular neuroscience · 2021Review
- The rises and falls of opsin genes in 59 ray-finned fish genomes and their implications for environmental adaptation.Scientific reports · 2017Article
- Chromophore supply rate-limits mammalian photoreceptor dark adaptation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Bleaching of mouse rods: microspectrophotometry and suction-electrode recording.The Journal of physiology · 2012Article
- Rod and cone visual pigments and phototransduction through pharmacological, genetic, and physiological approaches.The Journal of biological chemistry · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The visual pigment in vertebrate photoreceptors is a G protein-coupled receptor that consists of a protein, opsin, covalently attached to a chromophore, 11-cis-retinal. Activation of the visual pigment by light triggers a transduction cascade that produces experimentally measurable electrical responses in photoreceptors. The interactions between opsin and chromophore can be investigated with electrophysiologial recordings in intact amphibian and mouse rod and cone photoreceptor cells. Here we describe methods for substituting the native chromophore with various chromophore analogs to investigate how specific parts of the chromophore affect the signaling properties of the visual pigment and the function of photoreceptors. We also describe methods for genetically substituting the native rod opsin gene with cone opsins or with mutant rod opsins to investigate and compare their signaling properties. These methods are useful not only for understanding the relation between the properties of visual pigments and the function of photoreceptors but also for understanding the mechanisms by which mutations in rod opsin produce night blindness and other visual disorders.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.