ArticlePloS one2010
The effect on intracytoplasmic sperm injection outcome of genotype, male germ cell stage and freeze-thawing in mice.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Reverse transcriptase inhibitors enable the generation of fertile spermatids from fetal mouse testes in vitro.Communications biology · 2026Article
- Germ cell depletion using HSV-TK in mouse testes.Scientific reports · 2025Article
- Development of the membrane ceiling method for in vitro spermatogenesis.Scientific reports · 2025Article
- Spermatocyte injection into meiotic oocytes rescues diplotene, but not pachytene, arrest in azoospermic mutant mice.Human reproduction open · 2025Article
- Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors†.Biology of reproduction · 2021Article
- Improvement of a twice collection method of mouse oocytes by surgical operation.The Journal of reproduction and development · 2020Article
- Development of feline embryos produced by Piezo-actuated intracytoplasmic sperm injection of elongated spermatids.The Journal of reproduction and development · 2019Article
- Loss of H3K27me3 Imprinting in Somatic Cell Nuclear Transfer Embryos Disrupts Post-Implantation Development.Cell stem cell · 2018Article
- EPC1/TIP60-Mediated Histone Acetylation Facilitates Spermiogenesis in Mice.Molecular and cellular biology · 2017Article
- Progress and future prospect ofOncotarget · 2017Review
- Development of reproductive engineering techniques at the RIKEN BioResource Center.Experimental animals · 2017Review
- Complementary Critical Functions of Zfy1 and Zfy2 in Mouse Spermatogenesis and Reproduction.PLoS genetics · 2017Article
- Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Viable offspring obtained from Prm1-deficient sperm in mice.Scientific reports · 2016Article
- Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device.Scientific reports · 2016Article
- Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Overview of new developments in and the future of cryopreservation in the laboratory mouse.Mammalian genome : official journal of the International Mammalian Genome Society · 2012Review
- Article
- Hyperosmotic HEPES-CZB Enhances Post-ICSI Survival of C57BL/6N Mouse Oocytes.Reproductive medicine and biologyArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIntracytoplasmic sperm injection (ICSI) has been widely used to study the mechanisms of mammalian fertilization and to rescue male-factor infertility in humans and animals. However, very few systematic analyses have been conducted to define factors affecting the efficiency of ICSI. In this study, we undertook a large-scale series of ICSI experiments in mice to define the factors that might affect outcomes. METHODOLOGY/PRINCIPAL
findingsWe used a 5 x 3 x 2 factorial design with the following factors: mouse genotype (ICR, C57BL/6, DBA/2, C3H/He, and 129/Sv strains), type of male germ cells (epididymal sperm, elongated or round spermatids), and their freeze-thawing treatment. The efficiencies (parameters) of each developmental step were analyzed by three-way ANOVA (significance level P<0.01). The type of male germ cells affected all the four parameters observed: oocyte survival after injection, cleavage of oocytes, implantation, and birth of offspring. Genotype affected the oocyte survival, cleavage and birth rates, whereas freeze-thawing had no effects on any of the parameters. There were significant genotype/cell type interactions for oocyte survival and cleavage, indicating that they were determined by a combination of strain and germ cell maturity. Multiple comparisons revealed that spermatozoa and elongated spermatids gave better implantation and birth rates than did round spermatids, while spermatozoa and elongated spermatozoa were indistinguishable in their ability to support embryonic development. The best overall efficiency (birth rate per oocytes injected) was obtained with frozen-thawed DBA/2 strain elongated spermatids (23.2+/-4.2%). CONCLUSIONS/SIGNIFICANCE: The present study provides the first comprehensive information on ICSI using the mouse as a model and will contribute to the efficient use of materials, time, and efforts in biomedical research and clinics involving ICSI.
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