ArticleThe Journal of biological chemistry2010
A small GTPase activator protein interacts with cytoplasmic phytochromes in regulating root development.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- TheInternational journal of molecular sciences · 2024Article
- The ectomycorrhizal fungusFrontiers in microbiology · 2024Article
- Two Distinct Molecular Types of Phytochrome A in Plants: Evidence of Existence and Implications for Functioning.International journal of molecular sciences · 2023Review
- The Role of Light-Regulated Auxin Signaling in Root Development.International journal of molecular sciences · 2023Review
- Altering the balance between AOX1A and NDB2 expression affects a common set of transcripts in Arabidopsis.Frontiers in plant science · 2022Article
- RopGEF1 Plays a Critical Role in Polar Auxin Transport in Early Development.Plant physiology · 2017Article
- The RopGEF2-ROP7/ROP2 Pathway Activated by phyB Suppresses Red Light-Induced Stomatal Opening.Plant physiology · 2017Article
- Transcriptomes of Eight Arabidopsis thaliana Accessions Reveal Core Conserved, Genotype- and Organ-Specific Responses to Flooding Stress.Plant physiology · 2016Article
- Release of GTP Exchange Factor Mediated Down-Regulation of Abscisic Acid Signal Transduction through ABA-Induced Rapid Degradation of RopGEFs.PLoS biology · 2016Article
- PIF3 is involved in the primary root growth inhibition of Arabidopsis induced by nitric oxide in the light.Molecular plant · 2014Article
- Mesophyll-localized phytochromes gate stress- and light-inducible anthocyanin accumulation in Arabidopsis thaliana.Plant signaling & behavior · 2014Article
- AtPRK2 promotes ROP1 activation via RopGEFs in the control of polarized pollen tube growth.Molecular plant · 2013Article
- The circadian clock-associated small GTPase LIGHT INSENSITIVE PERIOD1 suppresses light-controlled endoreplication and affects tolerance to salt stress in Arabidopsis.Plant physiology · 2013Article
- Light-dependent, dark-promoted interaction between Arabidopsis cryptochrome 1 and phytochrome B proteins.The Journal of biological chemistry · 2012Article
- Spatial-specific regulation of root development by phytochromes in Arabidopsis thaliana.Plant signaling & behavior · 2011Article
- Root-localized phytochrome chromophore synthesis is required for photoregulation of root elongation and impacts root sensitivity to jasmonic acid in Arabidopsis.Plant physiology · 2011Article
- Coexpression-based clustering of Arabidopsis root genes predicts functional modules in early phosphate deficiency signaling.Plant physiology · 2011Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytochromes enable plants to sense light information and regulate developmental responses. Phytochromes interact with partner proteins to transmit light signals to downstream components for plant development. PIRF1 (phytochrome-interacting ROP guanine-nucleotide exchange factor (RopGEF 1)) functions as a light-signaling switch regulating root development through the activation of ROPs (Rho-like GTPase of plant) in the cytoplasm. In vitro pulldown and yeast two-hybrid assays confirmed the interaction between PIRF1 and phytochromes. PIRF1 interacted with the N-terminal domain of phytochromes through its conserved PRONE (plant-specific ROP nucleotide exchanger) region. PIRF1 also interacted with ROPs and activated them in a phytochrome-dependent manner. The Pr form of phytochrome A enhanced the RopGEF activity of PIRF1, whereas the Pfr form inhibited it. A bimolecular fluorescence complementation analysis demonstrated that PIRF1 was localized in the cytoplasm and bound to the phytochromes in darkness but not in light. PIRF1 loss of function mutants (pirf1) of Arabidopsis thaliana showed a longer root phenotype in the dark. In addition, both PIRF1 overexpression mutants (PIRF1-OX) and phytochrome-null mutants (phyA-211 and phyB-9) showed retarded root elongation and irregular root hair formation, suggesting that PIRF1 is a negative regulator of phytochrome-mediated primary root development. We propose that phytochrome and ROP signaling are interconnected through PIRF1 in regulating the root growth and development in Arabidopsis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.