ArticleThe Journal of biological chemistry2010
P-TEFb kinase complex phosphorylates histone H1 to regulate expression of cellular and HIV-1 genes.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Impact of chromatin on HIV-1 latency: a multi-dimensional perspective.Epigenetics & chromatin · 2025Review
- CDK9 keeps RNA polymerase II on track.Cellular and molecular life sciences : CMLS · 2021Review
- Block-And-Lock: New Horizons for a Cure for HIV-1.Viruses · 2020Review
- Differences in Transcriptional Dynamics Between T-cells and Macrophages as Determined by a Three-State Mathematical Model.Scientific reports · 2020Article
- Systematic analysis of linker histone PTM hotspots reveals phosphorylation sites that modulate homologous recombination and DSB repair.DNA repair · 2020Article
- Post-translational Modification-Based Regulation of HIV Replication.Frontiers in microbiology · 2018Review
- Chromatin Regulation and the Histone Code in HIV Latency .The Yale journal of biology and medicine · 2017Review
- Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation.Epigenetics & chromatin · 2017Article
- The pol II CTD: new twists in the tail.Nature structural & molecular biology · 2016Review
- HIV-1 Vpr reactivates latent HIV-1 provirus by inducing depletion of class I HDACs on chromatin.Scientific reports · 2016Article
- Gene activity in primary T cells infected with HIV89.6: intron retention and induction of genomic repeats.Retrovirology · 2015Article
- Transcriptional coactivator CIITA, a functional homolog of TAF1, has kinase activity.Biochimica et biophysica acta · 2013Article
- Cdk9 phosphorylates Pirh2 protein and prevents degradation of p53 protein.Cell cycle (Georgetown, Tex.) · 2013Article
- Use of ATP analogs to inhibit HIV-1 transcription.Virology · 2012Article
- Phosphorylation of histone H1 by P-TEFb is a necessary step in skeletal muscle differentiation.Journal of cellular physiology · 2012Article
- The control of HIV transcription: keeping RNA polymerase II on track.Cell host & microbe · 2011Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of HIV-1 genes depends on the RNA polymerase II kinase and elongation factor positive transcription elongation factor b (P-TEFb), the complex of cyclin T1 and CDK9. Recent evidence suggests that regulation of transcription by P-TEFb involves chromatin binding and modifying factors. To determine how P-TEFb may connect chromatin remodeling to transcription, we investigated the relationship between P-TEFb and histone H1. We identify histone H1 as a substrate for P-TEFb involved in cellular and HIV-1 transcription. We show that P-TEFb interacts with H1 and that P-TEFb inhibition by RNAi, flavopiridol, or dominant negative CDK9 expression correlates with loss of phosphorylation and mobility of H1 in vivo. Importantly, P-TEFb directs H1 phosphorylation in response to wild-type HIV-1 infection, but not Tat-mutant HIV-1 infection. Our results show that P-TEFb phosphorylates histone H1 at a specific C-terminal phosphorylation site. Expression of a mutant H1.1 that cannot be phosphorylated by P-TEFb also disrupts Tat transactivation in an HIV reporter cell line as well as transcription of the c-fos and hsp70 genes in HeLa cells. We identify histone H1 as a novel P-TEFb substrate, and our results suggest new roles for P-TEFb in both cellular and HIV-1 transcription.
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