ArticleDiabetes care2010
Homocysteinethiolactone and paraoxonase: novel markers of diabetic retinopathy.
Article in Diabetes care, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Unlocking Lactonase Enzymes as Biocatalysts for the Deracemisation of Chiral γ-Thiolactones.Angewandte Chemie (International ed. in English) · 2025Article
- Homocysteine Metabolites, Endothelial Dysfunction, and Cardiovascular Disease.International journal of molecular sciences · 2025Review
- The Implication of a Polymorphism in the Methylenetetrahydrofolate Reductase Gene in Homocysteine Metabolism and Related Civilisation Diseases.International journal of molecular sciences · 2023Review
- Gestational palmitic acid suppresses embryonic GATA-binding protein 4 signaling and causes congenital heart disease.Cell reports. Medicine · 2023Article
- Inhibiting MARSs reduces hyperhomocysteinemia-associated neural tube and congenital heart defects.EMBO molecular medicine · 2020Article
- Sex difference: an important issue to consider in epidemiological and clinical studies dealing with serum paraoxonase-1.Journal of clinical biochemistry and nutrition · 2019Article
- Article
- Commentary: Role of hyperhomocysteinemia in proliferative diabetic retinopathy: A case-control study.Indian journal of ophthalmology · 2018Article
- Remodeling of Retinal Architecture in Diabetic Retinopathy: Disruption of Ocular Physiology and Visual Functions by Inflammatory Gene Products and Pyroptosis.Frontiers in physiology · 2018Review
- Ocular distribution of antioxidant enzyme paraoxonase & its alteration in cataractous lens & diabetic retina.The Indian journal of medical research · 2017Article
- Lactonase Activity and Lipoprotein-Phospholipase AOxidative medicine and cellular longevity · 2017Article
- Levels of selected oxidative stress markers in the vitreous and serum of diabetic retinopathy patients.Molecular vision · 2015Article
- Human valacyclovir hydrolase/biphenyl hydrolase-like protein is a highly efficient homocysteine thiolactonase.PloS one · 2014Article
- Effects of betaine on body composition, performance, and homocysteine thiolactone.Journal of the International Society of Sports Nutrition · 2013Article
- Relationship between paraoxonase and homocysteine: crossroads of oxidative diseases.Archives of medical science : AMS · 2012Article
- Intravitreal homocysteine-thiolactone injection leads to the degeneration of multiple retinal cells, including photoreceptors.Molecular vision · 2011Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveParaoxonase (PON) exhibits esterase activity (PON-AREase) and lactonase activity (PON-HCTLase), which prevent LDL oxidation and detoxify homocysteine thiolactone (HCTL). The role of HCTL and PON-HCTLase as a risk factor for the microvascular complication in diabetic retinopathy at the level of vitreous has not been investigated. RESEARCH DESIGN AND
methodsUndiluted vitreous from patients with proliferative diabetic retinopathy (PDR) (n = 13) and macular hole (MH) (n = 8) was used to determine PON-HCTLase and PON-AREase activity spectrophotometrically. HCTL levels were detected by liquid chromatography-tandem mass spectrometry. In vitro studies were done in primary cultures of bovine retinal capillary endothelial cells (BRECs) to determine the dose- and time-dependent effect of HCTL and homocysteine (Hcys) on PON-HCTLase activity, as well as to determine mRNA expression of PON by RT-PCR.
resultsA significant increase in HCTL and PON-HCTLase activity was observed in PDR compared with MH (P = 0.036, P = 0.001), with a significant positive correlation between them (r = 0.77, P = 0.03). The in vitro studies on BRECs showed a dose- and time-dependent increase in the PON-HCTLase activity and mRNA expression of PON2 when exposed to HCTL and Hcys.
conclusionsThis is the first study showing elevated levels of vitreous HCTL and PON-HCTLase activity in PDR. These elevations are probably a protective effect to eliminate HCTL, which mediates endothelial cell dysfunction. Thus, vitreous levels of HCTL and PON activity can be markers of diabetic retinopathy. The bioinformatics analysis reveals that the structure and function of PON that can be modulated by hyperhomocysteinemia in PDR can affect the dual-enzyme activity of PON.
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