ArticleBiophysical journal2010
Millisecond timescale dynamics of human liver fatty acid binding protein: testing of its relevance to the ligand entry process.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Natural Fatty Acids as Dual ACE2-Inflammatory Modulators: Integrated Computational Framework for Pandemic Preparedness.International journal of molecular sciences · 2025Article
- A combined computational-biophysical approach to understanding fatty acid binding to FABP7.Biophysical journal · 2023Article
- Understanding FABP7 binding to fatty acid micelles and membranes.Biophysical journal · 2023Article
- Ligand Entry into Fatty Acid Binding Protein via Local Unfolding Instead of Gap Widening.Biophysical journal · 2020Article
- Retinoic Acid Binding Leads to CRABP2 Rigidification and Dimerization.Biochemistry · 2019Article
- Atomistic Insights into the Functional Instability of the Second Helix of Fatty Acid Binding Protein.Biophysical journal · 2019Article
- The Observation of Ligand-Binding-Relevant Open States of Fatty Acid Binding Protein by Molecular Dynamics Simulations and a Markov State Model.International journal of molecular sciences · 2019Article
- Coexistence of multiple minor states of fatty acid binding protein and their functional relevance.Scientific reports · 2016Article
- Review
- A New Method for Determining Structure Ensemble: Application to a RNA Binding Di-Domain Protein.Biophysical journal · 2016Article
- 13Cα CEST experiment on uniformly 13C-labeled proteins.Journal of biomolecular NMR · 2015Article
- Structural and functional interaction of fatty acids with human liver fatty acid-binding protein (L-FABP) T94A variant.The FEBS journal · 2014Article
- The human liver fatty acid binding protein T94A variant alters the structure, stability, and interaction with fibrates.Biochemistry · 2013Article
- Enhanced accuracy of kinetic information from CT-CPMG experiments by transverse rotating-frame spectroscopy.Journal of biomolecular NMR · 2013Article
- Rigidifying acyl carrier protein domain in iterative type I PKS CalE8 does not affect its function.Biophysical journal · 2012Article
- Probing the determinants of diacylglycerol binding affinity in the C1B domain of protein kinase Cα.Journal of molecular biology · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For over a decade, scientists have been attempting to know more about the conformational dynamics of fatty acid binding proteins (FABPs), to answer the puzzling question of how ligands could access the internalized binding site(s). Conformational exchange of FABPs on the microsecond to millisecond timescales has been found in many FABPs and offers an important hypothesis for the ligand entry mechanism. Despite the potential significance, the validity of this hypothesis has not been verified yet. In this study, the slow dynamics of human liver fatty acid binding protein (hLFABP) that was shown previously to be highly flexible on millisecond timescales was quantitatively characterized in detail. In addition, the interaction between hLFABP and 1,8-ANS was studied using NMR spectroscopy, and the kinetic rate of ANS association to hLFABP was measured. We believe the current result excludes the possibility that the intrinsic millisecond dynamics of hLFABP represents a critical conformational reorganization process required for ligand entry, but implies that it may represent the exchange between the apo-state and a state resembling the singly-bound conformation. Furthermore, we suggest these results show that the ligand-entry related functional dynamics could occur on the microsecond/submicrosecond timescales, highly encouraging future computational studies on this topic.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.