ArticleBiophysical journal2010
An electrostatic model for DNA surface hybridization.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 109 citations in OpenAlex.
- Chronocoulometric Quantification of DNA Surface Coverage on Electrochemically Functionalized MoSACS measurement science au · 2026Article
- Constant Temperature Electrochemical Biosensor for SNP Detection in Human Genomic DNA Based on DNA Melting Analysis.ACS sensors · 2025Article
- Electro-switchable addressing system for achieving repetitive random data access.Nucleic acids research · 2025Article
- Tuning Ion Current Rectifying Nanopipettes for Sensitive Detection of Methicillin-ResistantAnalytical chemistry · 2025Article
- Design and Validation of Specific Oligonucleotide Probes on Planar Magnetic Biosensors.Analytical chemistry · 2024Article
- Strategies in the optimization of DNA hybridization conditions and its role in electrochemical detection of dengue virus (DENV) using response surface methodology (RSM).RSC advances · 2023Article
- Rational Design of Porous Poly(ethylene glycol) Films as a Matrix for ssDNA Immobilization and Hybridization.Bioengineering (Basel, Switzerland) · 2022Article
- Article
- Article
- Messenger RNA enrichment using synthetic oligo(T) click nucleic acids.Chemical communications (Cambridge, England) · 2020Article
- Non-Langmuir Kinetics of DNA Surface Hybridization.Biophysical journal · 2020Article
- Immobilization Techniques for Aptamers on Gold Electrodes for the Electrochemical Detection of Proteins: A Review.Biosensors · 2020Review
- Anomalous Trends in Nucleic Acid-Based Electrochemical Biosensors with Nanoporous Gold Electrodes.Analytical chemistry · 2019Article
- The Role of Structural Enthalpy in Spherical Nucleic Acid Hybridization.Journal of the American Chemical Society · 2018Article
- Proximity does not contribute to activity enhancement in the glucose oxidase-horseradish peroxidase cascade.Nature communications · 2016Article
- Electrical Stimulus Controlled Binding/Unbinding of Human Thrombin-Aptamer Complex.Scientific reports · 2016Article
- Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy.Beilstein journal of nanotechnology · 2016Article
- Crowding-induced cooperativity in DNA surface hybridization.Scientific reports · 2015Article
- What controls the hybridization thermodynamics of spherical nucleic acids?Journal of the American Chemical Society · 2015Article
- Photo-attachment of biomolecules for miniaturization on wicking Si-nanowire platform.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA hybridization at surfaces is a crucial process for biomolecular detection, genotyping, and gene expression analysis. However, hybridization density and kinetics can be strongly inhibited by electric fields from the negatively charged DNA as the reaction proceeds. Here, we develop an electrostatic model to optimize hybridization density and kinetics as a function of DNA surface density, salt concentrations, and applied voltages. The electrostatic repulsion from a DNA surface layer is calculated numerically and incorporated into a modified Langmuir scheme, allowing kinetic suppression of hybridization. At the low DNA probe densities typically used in assays (<10(13)/cm(2)), electrostatics effects are largely screened and hybridization is completed with fast kinetics. However, higher hybridization densities can be achieved at intermediate DNA surface densities, albeit with slower kinetics. The application of positive voltages circumvents issues resulting from the very high DNA probe density, allowing highly enhanced hybridization densities and accelerated kinetics, and validating recent experimental measurements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.