ArticleBiophysical journal2010
Spatial mapping of the biomechanical properties of the pericellular matrix of articular cartilage measured in situ via atomic force microscopy.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
86 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Alteration in cartilage matrix stiffness as an indicator and modulator of osteoarthritis.Bioscience reports · 2024Pooled it
- A Systematic Review and Guide to Mechanical Testing for Articular Cartilage Tissue Engineering.Tissue engineering. Part C, Methods · 2019Pooled it
- Early Post‑Traumatic Osteoarthritis Features Greater Cartilage Extracellular Matrix Proteoglycan Loss Compared to the Pericellular Matrix.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Diels-Alder Click Chemistry as a Dynamic-Covalent Crosslinking Method in Spheroid-Encapsulating Hydrogels for Cartilage Engineering.Advanced healthcare materials · 2026Article
- Viscoelastic recovery time of chondrocytes from monolayer and alginate cultures.bioRxiv : the preprint server for biology · 2026Article
- A damaging mutation in COL6A3 alters the mechanobiologic response of chondrocytes derived from human induced pluripotent stem cells.Stem cell research & therapy · 2026Article
- Ultrafast crosslinking, strongly adhesiveBioactive materials · 2026Article
- MicroRNA-mediated mechanotransduction and chondrocyte differentiation in mesenchymal stem cells.Animal cells and systems · 2026Review
- Metabolomic Profiling and Characterization of a Novel 3D Culture System for Studying Chondrocyte Mechanotransduction.Cellular and molecular bioengineering · 2025Article
- Maternal high-fat high-sugar diet impairs bone quality and strength but not cartilage in aging mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Characterization of Composite Agarose-Collagen Hydrogels for Chondrocyte Culture.Annals of biomedical engineering · 2025Article
- Mesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures?Bioengineering (Basel, Switzerland) · 2024Article
- Heat conduction simulation of chondrocyte-embedded agarose gels suggests negligible impact of viscoelastic dissipation on temperature change.Journal of biomechanics · 2024Article
- Matrix stiffness aggravates osteoarthritis progression through H3K27me3 demethylation induced by mitochondrial damage.iScience · 2024Article
- Cartilage responses to inflammatory stimuli and adipose stem/stromal cell-derived conditioned medium: Results from an ex vivo model.Regenerative therapy · 2024Article
- HDAC6 inhibition regulates substrate stiffness-mediated inflammation signaling in chondrocytes.Acta biochimica et biophysica Sinica · 2023Article
- Advanced Mechanical Testing Technologies at the Cellular Level: The Mechanisms and Application in Tissue Engineering.Polymers · 2023Review
- Biomechanics of Chondrocytes and Chondrons in Healthy Conditions and Osteoarthritis: A Review of the Mechanical Characterisations at the Microscale.Biomedicines · 2023Review
- Article
- Atomic Force Microscopy Micro-Indentation Methods for Determining the Elastic Modulus of Murine Articular Cartilage.Sensors (Basel, Switzerland) · 2023Article
26 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
In articular cartilage, chondrocytes are surrounded by a narrow region called the pericellular matrix (PCM), which is biochemically, structurally, and mechanically distinct from the bulk extracellular matrix (ECM). Although multiple techniques have been used to measure the mechanical properties of the PCM using isolated chondrons (the PCM with enclosed cells), few studies have measured the biomechanical properties of the PCM in situ. The objective of this study was to quantify the in situ mechanical properties of the PCM and ECM of human, porcine, and murine articular cartilage using atomic force microscopy (AFM). Microscale elastic moduli were quantitatively measured for a region of interest using stiffness mapping, or force-volume mapping, via AFM. This technique was first validated by means of elastomeric models (polyacrylamide or polydimethylsiloxane) of a soft inclusion surrounded by a stiff medium. The elastic properties of the PCM were evaluated for regions surrounding cell voids in the middle/deep zone of sectioned articular cartilage samples. ECM elastic properties were evaluated in regions visually devoid of PCM. Stiffness mapping successfully depicted the spatial arrangement of moduli in both model and cartilage surfaces. The modulus of the PCM was significantly lower than that of the ECM in human, porcine, and murine articular cartilage, with a ratio of PCM to ECM properties of approximately 0.35 for all species. These findings are consistent with previous studies of mechanically isolated chondrons, and suggest that stiffness mapping via AFM can provide a means of determining microscale inhomogeneities in the mechanical properties of articular cartilage in situ.
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