ArticleBiophysical journal2010
ADP compartmentation analysis reveals coupling between pyruvate kinase and ATPases in heart muscle.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- A novel NaOncogene · 2024Article
- Rat and mouse cardiomyocytes show subtle differences in creatine kinase expression and compartmentalization.PloS one · 2023Article
- IOCBIO Kinetics: An open-source software solution for analysis of data traces.PLoS computational biology · 2020Article
- Respiration of permeabilized cardiomyocytes from mice: no sex differences, but substrate-dependent changes in the apparent ADP-affinity.Scientific reports · 2019Article
- Article
- Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.PloS one · 2018Article
- Metabolic compartmentation in rainbow trout cardiomyocytes: coupling of hexokinase but not creatine kinase to mitochondrial respiration.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2017Article
- The location of energetic compartments affects energetic communication in cardiomyocytes.Frontiers in physiology · 2014Article
- Tight coupling of Na+/K+-ATPase with glycolysis demonstrated in permeabilized rat cardiomyocytes.PloS one · 2014Article
- Unchanged mitochondrial organization and compartmentation of high-energy phosphates in creatine-deficient GAMT-/- mouse hearts.American journal of physiology. Heart and circulatory physiology · 2013Article
- Real-time determination of sarcomere length of a single cardiomyocyte during contraction.American journal of physiology. Cell physiology · 2013Article
- Molecular dynamics simulations of creatine kinase and adenine nucleotide translocase in mitochondrial membrane patch.The Journal of biological chemistry · 2012Article
- Analysis of molecular movement reveals latticelike obstructions to diffusion in heart muscle cells.Biophysical journal · 2012Article
- Sensitivity analysis of flux determination in heart by H₂ ¹⁸O -provided labeling using a dynamic Isotopologue model of energy transfer pathways.PLoS computational biology · 2012Article
- Permeabilized rat cardiomyocyte response demonstrates intracellular origin of diffusion obstacles.Biophysical journal · 2011Article
- Application of regularized Richardson-Lucy algorithm for deconvolution of confocal microscopy images.Journal of microscopy · 2011Article
- Analyzing the functional properties of the creatine kinase system with multiscale 'sloppy' modeling.PLoS computational biology · 2011Article
- Symbolic flux analysis for genome-scale metabolic networks.BMC systems biology · 2011Article
- Life of mice - development of cardiac energetics.The Journal of physiology · 2010Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cardiomyocytes have intracellular diffusion restrictions, which spatially compartmentalize ADP and ATP. However, the models that predict diffusion restrictions have used data sets generated in rat heart permeabilized fibers, where diffusion distances may be heterogeneous. This is avoided by using isolated, permeabilized cardiomyocytes. The aim of this work was to analyze the intracellular diffusion of ATP and ADP in rat permeabilized cardiomyocytes. To do this, we measured respiration rate, ATPase rate, and ADP concentration in the surrounding solution. The data were analyzed using mathematical models that reflect different levels of cell compartmentalization. In agreement with previous studies, we found significant diffusion restriction by the mitochondrial outer membrane and confirmed a functional coupling between mitochondria and a fraction of ATPases in the cell. In addition, our experimental data show that considerable activity of endogenous pyruvate kinase (PK) remains in the cardiomyocytes after permeabilization. A fraction of ATPases were inactive without ATP feedback by this endogenous PK. When analyzing the data, we were able to reproduce the measurements only with the mathematical models that include a tight coupling between the fraction of endogenous PK and ATPases. To our knowledge, this is the first time such a strong coupling of PK to ATPases has been demonstrated in permeabilized cardiomyocytes.
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