ArticleACS nano2010
Correlative fluorescence microscopy and scanning transmission electron microscopy of quantum-dot-labeled proteins in whole cells in liquid.
Article in ACS nano, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 87 citations in OpenAlex.
- Imaging of Hydrated and Living Cells in Transmission Electron Microscope: Summary, Challenges, and Perspectives.ACS nano · 2025Review
- Real-time visualisation of fast nanoscale processes during liquid reagent mixing by liquid cell transmission electron microscopy.Communications chemistry · 2025Article
- Transmission Electron Microscopy as a Powerful Tool to Investigate the Interaction of Nanoparticles with Subcellular Structures.International journal of molecular sciences · 2021Review
- Pyrene Excimer-Based Fluorescent Labeling of Cysteines Brought into Close Proximity by Protein Dynamics: ASEM-Induced Thiol-Ene Click Reaction for High Spatial Resolution CLEM.International journal of molecular sciences · 2020Article
- In situ monitoring of exopolymer-dependent Mn mineralization on bacterial surfaces.Science advances · 2020Article
- Liquid cell transmission electron microscopy and its applications.Royal Society open science · 2020Review
- Linear Chains of HER2 Receptors Found in the Plasma Membrane Using Liquid-Phase Electron Microscopy.Biophysical journal · 2018Article
- The role of electron irradiation history in liquid cell transmission electron microscopy.Science advances · 2018Article
- Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy.Applied and environmental microbiology · 2017Article
- Transmission electron microscopy for nanomedicine: novel applications for long-established techniques.European journal of histochemistry : EJH · 2016Review
- Article
- Dynamic nano-imaging of label-free living cells using electron beam excitation-assisted optical microscope.Scientific reports · 2015Article
- Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy.Journal of visualized experiments : JoVE · 2015Article
- Local variations of HER2 dimerization in breast cancer cells discovered by correlative fluorescence and liquid electron microscopy.Science advances · 2015Article
- Correlated light and electron microscopy: ultrastructure lights up!Nature methods · 2015Review
- Correlative electron and fluorescence microscopy of magnetotactic bacteria in liquid: toward in vivo imaging.Scientific reports · 2014Article
- Novel contrasting and labeling procedures for correlative microscopy of thawed cryosections.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Direct in situ observation of nanoparticle synthesis in a liquid crystal surfactant template.ACS nano · 2012Article
- Delivery of Quantum Dot-siRNA Nanoplexes in SK-N-SH Cells for BACE1 Gene Silencing and Intracellular Imaging.Molecular therapy. Nucleic acids · 2012Article
- Charged nanoparticle dynamics in water induced by scanning transmission electron microscopy.Langmuir : the ACS journal of surfaces and colloids · 2012Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Correlative fluorescence microscopy and transmission electron microscopy (TEM) is a state-of-the-art microscopy methodology to study cellular function, combining the functionality of light microscopy with the high resolution of electron microscopy. However, this technique involves complex sample preparation procedures due to its need for either thin sections or frozen samples for TEM imaging. Here, we introduce a novel correlative approach capable of imaging whole eukaryotic cells in liquid with fluorescence microscopy and with scanning transmission electron microscopy (STEM); there is no additional sample preparation necessary for the electron microscopy. Quantum dots (QDs) were bound to epidermal growth factor (EGF) receptors of COS7 fibroblast cells. Fixed whole cells in saline water were imaged with fluorescence microscopy and subsequently with STEM. The STEM images were correlated with fluorescence images of the same cellular regions. QDs of dimensions 7x12 nm were visible in a 5 microm thick layer of saline water, consistent with calculations. A spatial resolution of 3 nm was achieved on the QDs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.