ArticleJournal of industrial microbiology & biotechnology2010
Purification and characterization studies of a thermostable β-xylanase from Aspergillus awamori.
Article in Journal of industrial microbiology & biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 53 citations in OpenAlex.
- Exploring the potential of a new thermotolerant xylanase from3 Biotech · 2024Article
- Study of protease activity from Aspergillus awamori INCQS2B.361U2/1 extracellular fraction and modification of culture medium composition to isolate a novel aspartic protease.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production.Scientific reports · 2021Article
- Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutantBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Article
- Evaluation of biomass quality in short-rotation bamboo (Biotechnology for biofuels · 2017Article
- Lignocellulose conversion for biofuel: a new pretreatment greatly improves downstream biocatalytic hydrolysis of various lignocellulosic materials.Biotechnology for biofuels · 2015Article
- Bioethanol production from rice straw by popping pretreatment.Biotechnology for biofuels · 2013Article
- Characterization and constitutive expression of an acidic mesophilic endo-1,4-β-D-xylanohydrolase with high thermotolerance and catalytic efficiency in Pichia pastoris.World journal of microbiology & biotechnology · 2013Article
- Analysis of functional xylanases in xylan degradation by Aspergillus niger E-1 and characterization of the GH family 10 xylanase XynVII.SpringerPlus · 2013Article
- Efficient production of lignocellulolytic enzymes xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by the mutant strain Aspergillus awamori 2B.361 U2/1.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- Characterization and pH-dependent substrate specificity of alkalophilic xylanase from Bacillus alcalophilus.Journal of industrial microbiology & biotechnology · 2012Article
- Cloning, expression, and characterization of an alkaline thermostable GH11 xylanase from Thermobifida halotolerans YIM 90462T.Journal of industrial microbiology & biotechnology · 2012Article
- Sugarcane bagasse enzymatic hydrolysis: rheological data as criteria for impeller selection.Journal of industrial microbiology & biotechnology · 2011Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study presents data on the production, purification, and properties of a thermostable β-xylanase produced by an Aspergillus awamori 2B.361 U2/1 submerged culture using wheat bran as carbon source. Fractionation of the culture filtrate by membrane ultrafiltration followed by Sephacryl S-200 and Q-Sepharose chromatography allowed for the isolation of a homogeneous xylanase (PXII-1), which was 32.87 kDa according to MS analysis. The enzyme-specific activity towards soluble oat spelt xylan, which was found to be 490 IU/mg under optimum reaction conditions (50°C and pH 5.0-5.5), was 17-fold higher than that measured in the culture supernatant. Xylan reaction products were identified as xylobiose, xylotriose, and xylotetraose. K (m) values (mg ml(-1)) for soluble oat spelt and birchwood xylan were 11.8 and 9.45, respectively. Although PXII-1 showed 85% activity retention upon incubation at 50 °C and pH 5.0 for 20 days, incubation at pH 7.0 resulted in 50% activity loss within 3 days. PXII-1 stability at pH 7.0 was improved in the presence of 20 mM cysteine, which allowed for 85% activity retention for 25 days. This study on the production in high yields of a remarkably thermostable xylanase is of significance due to the central role that this class of biocatalyst shares, along with cellulases, for the much needed enzymatic hydrolysis of biomass. Furthermore, stable xylanases are important for the manufacture of paper, animal feed, and xylooligosaccharides.
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