ArticleThe Journal of general physiology2010
Frequency and release flux of calcium sparks in rat cardiac myocytes: a relation to RYR gating.
Article in The Journal of general physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Structure-based mechanism of RyR channel operation by calcium and magnesium ions.PLoS computational biology · 2025Article
- SAR296968, a Novel Selective NaBiomedicines · 2022Article
- Article
- In silico simulations reveal that RYR distribution affects the dynamics of calcium release in cardiac myocytes.The Journal of general physiology · 2021Article
- Magnesium Ions Moderate Calcium-Induced Calcium Release in Cardiac Calcium Release Sites by Binding to Ryanodine Receptor Activation and Inhibition Sites.Frontiers in physiology · 2021Article
- Structure and Function of the Human Ryanodine Receptors and Their Association with Myopathies-Present State, Challenges, and Perspectives.Molecules (Basel, Switzerland) · 2020Review
- Structural variability of dyads relates to calcium release in rat ventricular myocytes.Scientific reports · 2020Article
- Stochastic and deterministic approaches to modelling calcium release in cardiac myocytes at different spatial arrangements of ryanodine receptors.European biophysics journal : EBJ · 2019Article
- Calmodulin inhibition of human RyR2 channels requires phosphorylation of RyR2-S2808 or RyR2-S2814.Journal of molecular and cellular cardiology · 2019Article
- Calcium Signaling and Contractility in Cardiac Myocyte of Wolframin Deficient Rats.Frontiers in physiology · 2019Article
- Effects of rogue ryanodine receptors on CaRoyal Society open science · 2018Article
- The Interplay of Rogue and Clustered Ryanodine Receptors Regulates CaFrontiers in physiology · 2018Article
- The N-Terminal Region of the Ryanodine Receptor Affects Channel Activation.Frontiers in physiology · 2017Article
- Murine Electrophysiological Models of Cardiac Arrhythmogenesis.Physiological reviews · 2017Review
- Effects of Modified Parvalbumin EF-Hand Motifs on Cardiac Myocyte Contractile Function.Biophysical journal · 2016Article
- Examination of the Effects of Heterogeneous Organization of RyR Clusters, Myofibrils and Mitochondria on Ca2+ Release Patterns in Cardiomyocytes.PLoS computational biology · 2015Article
- Ryanodine receptor gating controls generation of diastolic calcium waves in cardiac myocytes.The Journal of general physiology · 2015Article
- Cardiac ryanodine receptor activation by a high Ca²⁺ store load is reversed in a reducing cytoplasmic redox environment.Journal of cell science · 2014Article
- Mechanisms of altered Ca²⁺ handling in heart failure.Circulation research · 2013Review
- Ca(2+) release events in cardiac myocytes up close: insights from fast confocal imaging.PloS one · 2013Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Cytosolic calcium concentration in resting cardiac myocytes locally fluctuates as a result of spontaneous microscopic Ca(2+) releases or abruptly rises as a result of an external trigger. These processes, observed as calcium sparks, are fundamental for proper function of cardiac muscle. In this study, we analyze how the characteristics of spontaneous and triggered calcium sparks are related to cardiac ryanodine receptor (RYR) gating. We show that the frequency of spontaneous sparks and the probability distribution of calcium release flux quanta of triggered sparks correspond quantitatively to predictions of an allosteric homotetrameric model of RYR gating. This model includes competitive binding of Ca(2+) and Mg(2+) ions to the RYR activation sites and allosteric interaction between divalent ion binding and channel opening. It turns out that at rest, RYRs are almost fully occupied by Mg(2+). Therefore, spontaneous sparks are most frequently evoked by random openings of the highly populated but rarely opening Mg(4)RYR and CaMg(3)RYR forms, whereas triggered sparks are most frequently evoked by random openings of the less populated but much more readily opening Ca(2)Mg(2)RYR and Ca(3)MgRYR forms. In both the spontaneous and the triggered sparks, only a small fraction of RYRs in the calcium release unit manages to open during the spark because of the limited rate of Mg(2+) unbinding. This mechanism clarifies the unexpectedly low calcium release flux during elementary release events and unifies the theory of calcium signaling in resting and contracting cardiac myocytes.
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