ArticleProceedings of the National Academy of Sciences of the United States of America2010
Pheromone-induced anisotropy in yeast plasma membrane phosphatidylinositol-4,5-bisphosphate distribution is required for MAPK signaling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed, 101 citations in OpenAlex.
- Molecular Mechanisms of Pathogenic Fungal Virulence Regulation by Cell Membrane Phospholipids.Journal of fungi (Basel, Switzerland) · 2025Review
- Regulation of yeast polarized exocytosis by phosphoinositide lipids.Cellular and molecular life sciences : CMLS · 2024Review
- Septin Organization and Dynamics for Budding Yeast Cytokinesis.Journal of fungi (Basel, Switzerland) · 2024Review
- The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast.PLoS biology · 2024Article
- A mechanism for exocyst-mediated tethering via Arf6 and PIP5K1C-driven phosphoinositide conversion.Current biology : CB · 2022Article
- Acetate and hypertonic stress stimulate vacuole membrane fission using distinct mechanisms.PloS one · 2022Article
- Heterologous Expression and Assembly of Human TLR Signaling Components inBiomolecules · 2021Article
- Lipid flippases in polarized growth.Current genetics · 2021Review
- A heat-sensitive Osh protein controls PI4P polarity.BMC biology · 2020Article
- Analysis of the roles of phosphatidylinositol-4,5-Molecular biology of the cell · 2019Article
- Regulation of TORC2 function and localization by Rab5 GTPases inCell cycle (Georgetown, Tex.) · 2019Review
- The interface between phosphatidylinositol transfer protein function and phosphoinositide signaling in higher eukaryotes.Journal of lipid research · 2019Review
- Tracking yeast pheromone receptor Ste2 endocytosis using fluorogen-activating protein tagging.Molecular biology of the cell · 2018Article
- Yeast mating: Ceramide acyl chain length matters?Cell cycle (Georgetown, Tex.) · 2018Article
- The Unsolved Problem of How Cells Sense Micron-Scale Curvature.Trends in biochemical sciences · 2017Review
- Many roads to symmetry breaking: molecular mechanisms and theoretical models of yeast cell polarity.Molecular biology of the cell · 2017Review
- Spatial and temporal signal processing and decision making by MAPK pathways.The Journal of cell biology · 2017Review
- A link between very long chain fatty acid elongation and mating-specific yeast cell cycle arrest.Cell cycle (Georgetown, Tex.) · 2017Article
- The final cut: cell polarity meets cytokinesis at the bud neck in S. cerevisiae.Cellular and molecular life sciences : CMLS · 2016Review
- Coordinate action of distinct sequence elements localizes checkpoint kinase Hsl1 to the septin collar at the bud neck in Saccharomyces cerevisiae.Molecular biology of the cell · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
During response of budding yeast to peptide mating pheromone, the cell becomes markedly polarized and MAPK scaffold protein Ste5 localizes to the resulting projection (shmoo tip). We demonstrated before that this recruitment is essential for sustained MAPK signaling and requires interaction of a pleckstrin homology (PH) domain in Ste5 with phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)] in the plasma membrane. Using fluorescently tagged high-affinity probes specific for PtdIns(4,5)P(2), we have now found that this phosphoinositide is highly concentrated at the shmoo tip in cells responding to pheromone. Maintenance of this strikingly anisotropic distribution of PtdIns(4,5)P(2), stable tethering of Ste5 at the shmoo tip, downstream MAPK activation, and expression of a mating pathway-specific reporter gene all require continuous function of the plasma membrane-associated PtdIns 4-kinase Stt4 and the plasma membrane-associated PtdIns4P 5-kinase Mss4 (but not the Golgi-associated PtdIns 4-kinase Pik1). Our observations demonstrate that PtdIns(4,5)P(2) is the primary determinant for restricting localization of Ste5 within the plasma membrane and provide direct evidence that an extracellular stimulus-evoked self-reinforcing mechanism generates a spatially enriched pool of PtdIns(4,5)P(2) necessary for the membrane anchoring and function of a signaling complex.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.