ArticleJournal of experimental botany2010
Proteomic changes associated with expression of a gene (ipt) controlling cytokinin synthesis for improving heat tolerance in a perennial grass species.
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 99 citations in OpenAlex.
- Gene Editing of Nicotiana benthamiana Architecture for Space-Efficient Production of Recombinant Proteins in Closed Environments.Plant biotechnology journal · 2026Article
- Integrated Transcriptomics and Metabolomics with Machine Learning Identify Flavonoids as Key Effectors in Wheat Root Thermotolerance.Plants (Basel, Switzerland) · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- De novo transcriptome assembly and gene expression analysis of Cnidium officinale under high-temperature conditions.BMC genomics · 2025Article
- Physiological and molecular characteristics associated with the anti-senescence inPhotosynthetica · 2024Article
- Identification and Expression Analysis of the Isopentenyl Transferase (IPT) Gene Family under Lack of Nitrogen Stress in Oilseed (Plants (Basel, Switzerland) · 2023Article
- Exogenous Kinetin Modulates ROS Homeostasis to Affect Heat Tolerance in Rice Seedlings.International journal of molecular sciences · 2023Article
- Modifications of Phytohormone Metabolism Aimed at Stimulation of Plant Growth, Improving Their Productivity and Tolerance to Abiotic and Biotic Stress Factors.Plants (Basel, Switzerland) · 2022Review
- Cytokinin and abiotic stress tolerance -What has been accomplished and the way forward?Frontiers in genetics · 2022Review
- Exogenous phytohormones in the regulation of growth and development of cereals under abiotic stresses.Molecular biology reports · 2022Review
- Isopentenyltransferases as master regulators of crop performance: their function, manipulation, and genetic potential for stress adaptation and yield improvement.Plant biotechnology journal · 2021Review
- Leaf senescence: progression, regulation, and application.Molecular horticulture · 2021Review
- Research Progress on the Roles of Cytokinin in Plant Response to Stress.International journal of molecular sciences · 2020Review
- Plant Hormone-Mediated Regulation of Heat Tolerance in Response to Global Climate Change.Frontiers in plant science · 2020Review
- The Mode of Cytokinin Functions Assisting Plant Adaptations to Osmotic Stresses.Plants (Basel, Switzerland) · 2019Review
- Candidate Genes and Molecular Markers Correlated to Physiological Traits for Heat Tolerance in Fine Fescue Cultivars.International journal of molecular sciences · 2018Article
- Ethylene Role in Plant Growth, Development and Senescence: Interaction with Other Phytohormones.Frontiers in plant science · 2017Review
- Candidate genes and molecular markers associated with heat tolerance in colonial Bentgrass.PloS one · 2017Article
- Stimulation of ipt overexpression as a tool to elucidate the role of cytokinins in high temperature responses of Arabidopsis thaliana.Journal of experimental botany · 2016Article
- Prediction of protein targets of kinetin using in silico and in vitro methods: a case study on spinach seed germination mechanism.Journal of chemical biology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinins (CKs) are known to regulate leaf senescence and affect heat tolerance, but mechanisms underlying CK regulation of heat tolerance are not well understood. A comprehensive proteomic study was conducted to identify proteins altered by the expression of the adenine isopentenyl transferase (ipt) gene controlling CK synthesis and associated with heat tolerance in transgenic plants for a C(3) perennial grass species, Agrostis stolonifera. Transgenic plants with two different inducible promoters (SAG12 and HSP18) and a null transformant (NT) containing the vector without ipt were exposed to 20 degrees C (control) or 35 degrees C (heat stress) in growth chambers. Two-dimensional electrophoresis and mass spectrometry analysis were performed to identify protein changes in leaves and roots in response to ipt expression under heat stress. Transformation with ipt resulted in protein changes in leaves and roots involved in multiple functions, particularly in energy metabolism, protein destination and storage, and stress defence. The abundance levels of six leaf proteins (enolase, oxygen-evolving enhancer protein 2, putative oxygen-evolving complex, Rubisco small subunit, Hsp90, and glycolate oxidase) and nine root proteins (Fd-GOGAT, nucleotide-sugar dehydratase, NAD-dependent isocitrate dehydrogenase, ferredoxin-NADP reductase precursor, putative heterogeneous nuclear ribonucleoprotein A2, ascorbate peroxidase, dDTP-glucose 4-6-dehydratases-like protein, and two unknown proteins) were maintained or increased in at least one ipt transgenic line under heat stress. The diversity of proteins altered in transgenic plants in response to heat stress suggests a regulatory role for CKs in various metabolic pathways associated with heat tolerance in C(3) perennial grass species.
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