ReviewJournal of proteomics2010
Identifying components of protein complexes in C. elegans using co-immunoprecipitation and mass spectrometry.
Review in Journal of proteomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Screening and identification of protein 29 ofFrontiers in cellular and infection microbiology · 2025Article
- Identification of the Beta Subunit Fas1p of Fatty Acid Synthetase as an Interacting Partner of Yeast Calcium/Calmodulin-Dependent Protein Kinase Cmk2p Through Mass Spectrometry Analysis.Applied biochemistry and biotechnology · 2024Article
- Interrogating data-independent acquisition LC-MS/MS for affinity proteomics.Journal of proteins and proteomics · 2024Article
- Orthogonal Enzyme-Substrate Design Strategy for Discovery of Human Protein Palmitoyltransferase Substrates.Journal of the American Chemical Society · 2023Article
- Chromatin and non-chromatin immunoprecipitations to capture protein-protein and protein-nucleic acid interactions in living cells.Methods (San Diego, Calif.) · 2023Article
- Serum irisin levels are decreased in patients with sepsis, and exogenous irisin suppresses ferroptosis in the liver of septic mice.Clinical and translational medicine · 2020Article
- Increased talin-vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- Isolation and Identification of Protein L-Isoaspartate-O-Methyltransferase (PIMT) Interacting Proteins in Salmonella Typhimurium.Current microbiology · 2020Article
- Mass Spectrometry Advances and Perspectives for the Characterization of Emerging Adoptive Cell Therapies.Molecules (Basel, Switzerland) · 2020Review
- Protein Interface Complementarity and Gene Duplication Improve Link Prediction of Protein-Protein Interaction Network.Frontiers in genetics · 2020Article
- A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large.BMC biology · 2016Article
- Quantification of protein interaction kinetics in a micro droplet.The Review of scientific instruments · 2015Article
- Next-generation technologies for multiomics approaches including interactome sequencing.BioMed research international · 2015Review
- Protein-protein interaction detection: methods and analysis.International journal of proteomics · 2014Review
- Worming our way in and out of the Caenorhabditis elegans germline and developing embryo.Traffic (Copenhagen, Denmark) · 2013Review
- Effective identification of Akt interacting proteins by two-step chemical crosslinking, co-immunoprecipitation and mass spectrometry.PloS one · 2013Article
- Current approaches on viral infection: proteomics and functional validations.Frontiers in microbiology · 2012Article
- Mass spectrometry based proteomic studies on viruses and hosts--a review.Analytica chimica acta · 2011Review
- Cooperative transcription factor associations discovered using regulatory variation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Stable isotope labeling with amino acids in cell culture based mass spectrometry approach to detect transient protein interactions using substrate trapping.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Mass spectrometry-based proteomics is rapidly becoming an essential tool for biologists. One of the most common applications is identifying the components of protein complexes isolated by co-immunoprecipitation. In this review, we discuss the co-immunoprecipitation, mass spectrometry and data analysis techniques that have been used successfully to define protein complexes in C. elegans research. In this discussion, two strategies emerged. One approach is to use stringent biochemical purification methods and attempt to identify a small number of complex components with a high degree of certainty based on MS data. A second approach is to use less stringent purification and identification parameters, and ultimately test a longer list of potential binding partners in biological validation assays. This should provide a useful guide for biologists planning proteomic experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.