ArticleCell proliferation2010
Multilineage differentiation of dental follicle cells and the roles of Runx2 over-expression in enhancing osteoblast/cementoblast-related gene expression in dental follicle cells.
Article in Cell proliferation, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 60 citations in OpenAlex.
- The Role of Protein Kinase C During the Differentiation of Stem and Precursor Cells into Tissue Cells.Biomedicines · 2024Review
- CA1 Modulates the Osteogenic Differentiation of Dental Follicle Stem Cells by Activating the BMP Signaling Pathway In Vitro.Tissue engineering and regenerative medicine · 2024Article
- Clinical application prospects and transformation value of dental follicle stem cells in oral and neurological diseases.World journal of stem cells · 2023Review
- Priming strategies for controlling stem cell fate: Applications and challenges in dental tissue regeneration.World journal of stem cells · 2021Review
- Eruption of Bioengineered Teeth: A New Approach Based on a Polycaprolactone Biomembrane.Nanomaterials (Basel, Switzerland) · 2021Article
- Transcriptome analysis of the transdifferentiation of canine BMSCs into insulin producing cells.BMC genomics · 2021Article
- The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells.Nanomaterials (Basel, Switzerland) · 2020Article
- Human Oral Stem Cells, Biomaterials and Extracellular Vesicles: A Promising Tool in Bone Tissue Repair.International journal of molecular sciences · 2019Review
- NFIC promotes the vitality and osteogenic differentiation of rat dental follicle cells.Journal of molecular histology · 2019Article
- Dental Follicle Cells: Roles in Development and Beyond.Stem cells international · 2019Review
- Nkd2 promotes the differentiation of dental follicle stem/progenitor cells into osteoblasts.International journal of molecular medicine · 2018Article
- A novel hydrogel scaffold for periodontal ligament stem cells.Interventional medicine & applied science · 2018Article
- Micro/Nano Structural Tantalum Coating for Enhanced Osteogenic Differentiation of Human Bone Marrow Stem Cells.Materials (Basel, Switzerland) · 2018Article
- Cellular behaviours of bone marrow-derived mesenchymal stem cells towards pristine graphene oxide nanosheets.Cell proliferation · 2017Article
- Regulatory effects of bone morphogenetic protein-4 on tumour necrosis factor-α-suppressed Runx2 and osteoprotegerin expression in cementoblasts.Cell proliferation · 2017Article
- Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts.BMC cell biology · 2017Article
- Odontogenic differentiation and biomineralization potential of dental pulp stem cells inside Mg-based bioceramic scaffolds under low-level laser treatment.Lasers in medical science · 2017Article
- RUNX2 Mutation Impairs 1α,25-Dihydroxyvitamin D3 mediated Osteoclastogenesis in Dental Follicle Cells.Scientific reports · 2016Article
- Stem cells: Sources, and regenerative therapies in dental research and practice.World journal of stem cells · 2015Review
- The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs).Archives of oral biology · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesDental follicle cells (DFCs) provide the origin of periodontal tissues, and Runx2 is essential for bone formation and tooth development. In this study, pluripotency of DFCs was evaluated and effects of Runx2 on them were investigated. MATERIALS AND
methodsThe DFCs were induced to differentiate towards osteoblasts, adipocytes or chondrocytes, and alizarin red staining, oil red O staining or alcian blue staining was performed to reveal the differentiated states. Bone marrow stromal cells (BMSCs) and primary mouse fibroblasts served as controls. DFCs were also infected with recombinant retroviruses encoding either full-length Runx2 or mutant Runx2 without the VWRPY motif. Western blot analysis, real-time real time RT-PCR and in vitro mineralization assay were performed to evaluate the effects of full-length Runx2 or mutant Runx2 on osteogenic/cementogenic differentiation of the cells.
resultsThe above-mentioned staining methods demonstrated that DFCs were successfully induced to differentiate towards osteoblasts, adipocytes or chondrocytes respectively, confirming the existence of pluripotent mesenchymal stem cells in dental follicle tissues. However, staining intensity in DFC cultures was weaker than in BMSC cultures. Real-time PCR analysis indicated that mutant Runx2 induced a more pronounced increase in expression levels of OC, OPN, Col I and CP23 than full-length Runx2. Mineralization assay also showed that mutant Runx2 increased mineralization nodule formation more prominently than full-length Runx2.
conclusionsMultipotent DFCs can be induced to differentiate towards osteoblasts, adipocytes or chondrocytes in vitro. Runx2 over-expression up-regulated expression levels of osteoblast/cementoblast-related genes and in vitro enhanced osteogenic differentiation of DFCs. In addition, mutant Runx2-induced changes in DFCs were more prominent than those induced by full-length Runx2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.