ArticleThe Plant journal : for cell and molecular biology2010
Identification of protein stability determinants in chloroplasts.
Article in The Plant journal : for cell and molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 115 citations in OpenAlex.
- Sustainable production of myoglobin meat protein in plant chloroplasts.Frontiers in plant science · 2026Article
- Intra-chloroplast proteases: A holistic network view of chloroplast proteolysis.The Plant cell · 2024Review
- Red Rubiscos and opportunities for engineering green plants.Journal of experimental botany · 2023Review
- Chloroplast proteostasis: A story of birth, life, and death.Plant communications · 2023Review
- Understanding protein import in diverse non-green plastids.Frontiers in genetics · 2023Review
- Tobacco Plastid Transformation as Production Platform of Lytic Polysaccharide MonoOxygenase Auxiliary Enzymes.International journal of molecular sciences · 2022Article
- Plastid Transformation: New Challenges in the Circular Economy Era.International journal of molecular sciences · 2022Review
- Plant-produced recombinant cytokines IL-37b and IL-38 modulate inflammatory response from stimulated human PBMCs.Scientific reports · 2022Article
- Expression strategies for the efficient synthesis of antimicrobial peptides in plastids.Nature communications · 2022Article
- Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Characterization of the SARS-CoV-2 coronavirus X4-like accessory protein.The Egyptian journal of medical human genetics · 2021Article
- Overexpression of the recombinant human interferon-beta (Biotechnologia · 2021Article
- Evaluation of lettuce chloroplast and soybean cotyledon as platforms for production of functional bone morphogenetic protein 2.Transgenic research · 2019Article
- A highly efficient sulfadiazine selection system for the generation of transgenic plants and algae.Plant biotechnology journal · 2019Article
- Recent Advances and Current Challenges in Synthetic Biology of the Plastid Genetic System and Metabolism.Plant physiology · 2019Review
- High-level production of single chain monellin mutants with enhanced sweetness and stability in tobacco chloroplasts.Planta · 2018Article
- High-level expression of the HIV entry inhibitor griffithsin from the plastid genome and retention of biological activity in dried tobacco leaves.Plant molecular biology · 2018Article
- Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED1.Plant physiology · 2018Article
- Temporal Proteomics of Inducible RNAi Lines of Clp Protease Subunits Identifies Putative Protease Substrates.Plant physiology · 2018Article
- Real-time detection of N-end rule-mediated ubiquitination via fluorescently labeled substrate probes.The New phytologist · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although chloroplast protein stability has long been recognised as a major level of post-translational regulation in photosynthesis and gene expression, the factors determining protein stability in plastids are largely unknown. Here, we have identified stability determinants in vivo by producing plants with transgenic chloroplasts that express a reporter protein whose N- and C-termini were systematically modified. We found that major stability determinants are located in the N-terminus. Moreover, testing of all 20 amino acids in the position after the initiator methionine revealed strong differences in protein stability and indicated an important role of the penultimate N-terminal amino acid residue in determining the protein half life. We propose that the stability of plastid proteins is largely determined by three factors: (i) the action of methionine aminopeptidase (the enzyme that removes the initiator methionine and exposes the penultimate N-terminal amino acid residue), (ii) an N-end rule-like protein degradation pathway, and (iii) additional sequence determinants in the N-terminal region.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.