ArticleMolecular microbiology2010
Metabolic shutdown in Escherichia coli cells lacking the outer membrane channel TolC.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 69 citations in OpenAlex.
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- Beyond antibiotic resistance: evidence for resistance-nodulation-division (RND) efflux pumps as virulence determinants.Microbiology and molecular biology reviews : MMBR · 2025Review
- Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters.Science advances · 2025Article
- Characterization of a novel glycocin from a thermophile.bioRxiv : the preprint server for biology · 2025Article
- Rapid identification of key antibiotic resistance genes iniScience · 2025Article
- A historical perspective on the multifunctional outer membrane channel protein TolC in Escherichia coli.npj antimicrobials and resistance · 2025Review
- Physiological Effects of TolC-Dependent Multidrug Efflux Pumps in Escherichia coli: Impact on Motility and Growth Under Stress Conditions.MicrobiologyOpen · 2024Article
- Unveiling the impact ofInfection and immunity · 2024Article
- The metabolic slowdown caused by the deletion ofAntimicrobial agents and chemotherapy · 2024Article
- Total synthesis and mechanism of action of the antibiotic armeniaspirol A.Chemical science · 2021Article
- Drug Permeation against Efflux by Two Transporters.ACS infectious diseases · 2020Article
- A General Workflow for Characterization of Nernstian Dyes and Their Effects on Bacterial Physiology.Biophysical journal · 2020Article
- The Rho-Dependent Transcription Termination Is Involved in Broad-Spectrum Antibiotic Susceptibility inFrontiers in microbiology · 2020Article
- New Functional Criterion for Evaluation of Homologous MDR Pumps.Frontiers in microbiology · 2020Article
- Contributions of TolC Orthologs toInfection and immunity · 2019Article
- Impaired Efflux of the Siderophore Enterobactin Induces Envelope Stress inFrontiers in microbiology · 2019Article
- Breaking the Permeability Barrier of Escherichia coli by Controlled Hyperporination of the Outer Membrane.Antimicrobial agents and chemotherapy · 2016Article
- Type I Protein Secretion-Deceptively Simple yet with a Wide Range of Mechanistic Variability across the Family.EcoSal Plus · 2016Review
- Involvement of Antibiotic Efflux Machinery in Glutathione-Mediated Decreased Ciprofloxacin Activity in Escherichia coli.Antimicrobial agents and chemotherapy · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The outer membrane channel TolC is a key component of multidrug efflux and type I secretion transporters in Escherichia coli. Mutational inactivation of TolC renders cells highly susceptible to antibiotics and leads to defects in secretion of protein toxins. Despite impairment of various transport functions, no growth defects were reported in cells lacking TolC. Unexpectedly, we found that the loss of TolC notably impairs cell division and growth in minimal glucose medium. The TolC-dependent phenotype was further exacerbated by the loss of ygiB and ygiC genes expressed in the same operon as tolC and their homologues yjfM and yjfC located elsewhere on the chromosome. Our results show that this growth deficiency is caused by depletion of the critical metabolite NAD(+) and high NADH/NAD(+) ratios. The increased amounts of PspA and decreased rates of NADH oxidation in Delta tolC membranes indicated stress on the membrane and dissipation of a proton motive force. We conclude that inactivation of TolC triggers metabolic shutdown in E. coli cells grown in minimal glucose medium. The Delta tolC phenotype is partially rescued by YgiBC and YjfMC, which have parallel functions independent from TolC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.