ArticleJournal of biomolecular NMR2010
13C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides.
Article in Journal of biomolecular NMR, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- NMR characterisation of the antibiotic resistance-mediating 32mer RNA from the 23S ribosomal RNA.Biomolecular NMR assignments · 2025Article
- OptimizedInternational journal of molecular sciences · 2025Article
- Relaxation Optimized Heteronuclear Experiments for Extending the Size Limit of RNA Nuclear Magnetic Resonance.Journal of the American Chemical Society · 2025Article
- Paramagnetic NMR to study iron sulfur proteins:Journal of biomolecular NMR · 2023Article
- Primary Structure of Glycans by NMR Spectroscopy.Chemical reviews · 2023Review
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- Mechanisms of coherent re-arrangement for long-lived spin order.Magnetic resonance (Gottingen, Germany) · 2021Review
- More than Proton Detection-New Avenues for NMR Spectroscopy of RNA.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Review
- Combining asymmetric 13C-labeling and isotopic filter/edit NOESY: a novel strategy for rapid and logical RNA resonance assignment.Nucleic acids research · 2017Article
- Evaluation ofJournal of biomolecular NMR · 2017Article
- Direct ¹³C-detected NMR experiments for mapping and characterization of hydrogen bonds in RNA.Journal of biomolecular NMR · 2016Article
- Nucleotide-type chemical shift assignment of the encapsulated 40 kbp dsDNA in intact bacteriophage T7 by MAS solid-state NMR.Journal of biomolecular NMR · 2014Article
- NMR structure analysis of uniformly 13C-labeled carbohydrates.Journal of biomolecular NMR · 2014Article
- Improved NMR experiments with ¹³C-isotropic mixing for assignment of aromatic and aliphatic side chains in labeled proteins.Journal of biomolecular NMR · 2014Article
- Cell-free expressed bacteriorhodopsin in different soluble membrane mimetics: biophysical properties and NMR accessibility.Structure (London, England : 1993) · 2013Article
- Improving the chemical shift dispersion of multidimensional NMR spectra of intrinsically disordered proteins.Journal of biomolecular NMR · 2013Article
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7 authors.
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Abstract
We present here a set of (13)C-direct detected NMR experiments to facilitate the resonance assignment of RNA oligonucleotides. Three experiments have been developed: (1) the (H)CC-TOCSY-experiment utilizing a virtual decoupling scheme to assign the intraresidual ribose (13)C-spins, (2) the (H)CPC-experiment that correlates each phosphorus with the C4' nuclei of adjacent nucleotides via J(C,P) couplings and (3) the (H)CPC-CCH-TOCSY-experiment that correlates the phosphorus nuclei with the respective C1',H1' ribose signals. The experiments were applied to two RNA hairpin structures. The current set of (13)C-direct detected experiments allows direct and unambiguous assignment of the majority of the hetero nuclei and the identification of the individual ribose moieties following their sequential assignment. Thus, (13)C-direct detected NMR methods constitute useful complements to the conventional (1)H-detected approach for the resonance assignment of oligonucleotides that is often hindered by the limited chemical shift dispersion. The developed methods can also be applied to large deuterated RNAs.
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