ArticlePloS one2010
Proinflammatory phenotype and increased caveolin-1 in alveolar macrophages with silenced CFTR mRNA.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 49 citations in OpenAlex.
- Alveolar Macrophages: Development, Respiratory Homeostasis, Signal Pathways, Diseases, and Therapeutic Opportunities.MedComm · 2026Review
- Review
- Comparative effects of CFTR modulators on phagocytic, metabolic and inflammatory profiles of CF and nonCF macrophages.Scientific reports · 2023Article
- Exploring YAP1-centered networks linking dysfunctional CFTR to epithelial-mesenchymal transition.Life science alliance · 2022Article
- CFTR Protein: Not Just a Chloride Channel?Cells · 2021Review
- Caveolin-1 Alleviates Acetaminophen-Induced Fat Accumulation in Non-Alcoholic Fatty Liver Disease by Enhancing Hepatic Antioxidant AbilityFrontiers in pharmacology · 2021Article
- Consequences of CRISPR-Cas9-Mediated CFTR Knockout in Human Macrophages.Frontiers in immunology · 2020Article
- Cystic Fibrosis Inflammation: Hyperinflammatory, Hypoinflammatory, or Both?American journal of respiratory cell and molecular biology · 2019Article
- CFTR regulates B cell activation and lymphoid follicle development.Respiratory research · 2019Article
- Is cellular senescence involved in cystic fibrosis?Respiratory research · 2019Review
- CFTR prevents neuronal apoptosis following cerebral ischemia reperfusion via regulating mitochondrial oxidative stress.Journal of molecular medicine (Berlin, Germany) · 2018Article
- CFTR protects against vascular inflammation and atherogenesis in apolipoprotein E-deficient mice.Bioscience reports · 2017Article
- Monocyte derived macrophages from CF pigs exhibit increased inflammatory responses at birth.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2017Article
- Neutrophil extracellular traps and the dysfunctional innate immune response of cystic fibrosis lung disease: a review.Journal of inflammation (London, England) · 2017Review
- Article
- X-Box-Binding Protein 1 and Innate Immune Responses of Human Cystic Fibrosis Alveolar Macrophages.American journal of respiratory and critical care medicine · 2015Article
- Role of caveolin-3 in lymphocyte activation.Life sciences · 2015Article
- CFTR Knockdown induces proinflammatory changes in intestinal epithelial cells.Journal of inflammation (London, England) · 2015Article
- Second-hand cigarette smoke impairs bacterial phagocytosis in macrophages by modulating CFTR dependent lipid-rafts.PloS one · 2015Article
- Current concepts: host-pathogen interactions in cystic fibrosis airways disease.European respiratory review : an official journal of the European Respiratory Society · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The inflammatory milieu in the respiratory tract in cystic fibrosis (CF) has been linked to the defective expression of the cystic transmembrane regulator (CFTR) in epithelial cells. Alveolar macrophages (AM), important contibutors to inflammatory responses in the lung, also express CFTR. The present study analyzes the phenotype of human AM with silenced CFTR. Expression of CFTR mRNA and the immature form of the CFTR protein decreased 100-fold and 5.2-fold, respectively, in AM transfected with a CFTR specific siRNA (CFTR-siRNA) compared to controls. Reduction of CFTR expression in AM resulted in increased secretion of IL-8, increased phosphorylation of NF-kappaB, a positive regulator of IL-8 expression, and decreased expression of IkappaB-alpha, the inhibitory protein of NF-kappaB activation. AM with silenced CFTR expression also showed increased apoptosis. We hypothesized that caveolin-1 (Cav1), a membrane protein that is co-localized with CFTR in lipid rafts and that is related to inflammation and apoptosis in macrophages, may be affected by decreased CFTR expression. Messenger RNA and protein levels of Cav1 were increased in AM with silenced CFTR. Expression and transcriptional activity of sterol regulatory element binding protein (SREBP), a negative transcriptional regulator of Cav1, was decreased in AM with silenced CFTR, but total and free cholesterol mass did not change. These findings indicate that silencing of CFTR in human AM results in an inflammatory phenotype and apoptosis, which is associated to SREBP-mediated regulation of Cav1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.