ArticleCell adhesion & migration
Leupaxin is similar to paxillin in focal adhesion targeting and tyrosine phosphorylation but has distinct roles in cell adhesion and spreading.
Article in Cell adhesion & migration. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Nanotopography-Mediated Mechanotransduction Enhances hBMSCs Adhesion on TiOJournal of functional biomaterials · 2026Article
- The Leupaxin/HDAC6/EGR2 axis facilitates breast cancer progression by enhancing macrophage M2 polarization.Cell biology and toxicology · 2025Article
- Actin Binding to the BAR Domain and Arf GAP Activity of ASAP1 Coordinately Control Actin Stress Fibers and Focal Adhesions.Biology of the cell · 2025Article
- Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG.Journal of molecular and cellular cardiology · 2021Article
- Leupaxin Expression Is Dispensable for B Cell Immune Responses.Frontiers in immunology · 2020Article
- A kindlin-3-leupaxin-paxillin signaling pathway regulates podosome stability.The Journal of cell biology · 2019Article
- A component overlapping attribute clustering (COAC) algorithm for single-cell RNA sequencing data analysis and potential pathobiological implications.PLoS computational biology · 2019Article
- Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI-1 cells.Oncology letters · 2019Article
- RNAi screening identifies a mechanosensitive ROCK-JAK2-STAT3 network central to myofibroblast activation.Journal of cell science · 2018Article
- Paxillin: a crossroad in pathological cell migration.Journal of hematology & oncology · 2017Review
- Tumour-suppressor microRNAs regulate ovarian cancer cell physical properties and invasive behaviour.Open biology · 2016Article
- The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network.The Journal of biological chemistry · 2016Article
- Structural Basis for the Interaction between Pyk2-FAT Domain and Leupaxin LD Repeats.Biochemistry · 2016Article
- Schizophrenia interactome with 504 novel protein-protein interactions.NPJ schizophrenia · 2016Article
- Evolution and Expression of Paxillin Genes in Teleost Fish.PloS one · 2016Article
- Article
- The Arf6 GTPase-activating proteins ARAP2 and ACAP1 define distinct endosomal compartments that regulate integrin α5β1 traffic.The Journal of biological chemistry · 2014Article
- N-ethylmaleimide-sensitive factor attachment protein α (αSNAP) regulates matrix adhesion and integrin processing in human epithelial cells.The Journal of biological chemistry · 2014Article
- ARAP2 signals through Arf6 and Rac1 to control focal adhesion morphology.The Journal of biological chemistry · 2013Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Focal adhesion (FA) formation is induced by extracellular matrix-stimulated integrin clustering and activation of receptors for diffusible factors. Leupaxin (LPXN) is a member of the paxillin family of FA proteins expressed in many cancer cell lines. We found activation of gastrin-releasing peptide receptor (GRPr) by bombesin (BN) stimulated LPXN translocation from cytoplasm to FAs. Using mutagenesis, we identified LIM3 as the primary FA targeting domain for LPXN and showed BN-induced LPXN tyrosine phosphorylation on residues 22, 62 and 72. A LIM3 point mutant of LPXN failed to target to FAs and had no BN-stimulated tyrosine phosphorylation. Conversely, a non-phosphorylatable mutant (Y22/62/72F) translocated to FAs after BN addition. Stimulation of FA formation using vinblastine also induced LPXN translocation and tyrosine phosphorylation. Therefore, dynamic LPXN tyrosine phosphorylation requires translocation to FAs. LPXN and paxillin had opposite roles in adhesion to collagen I (CNI) in MDA-MB-231 breast cancer cells. LPXN siRNA stimulated whereas paxillin siRNA inhibited cell adhesion. Knockdown of both LPXN and paxillin behaved similarly to paxillin knockdown alone, suggesting LPXN’s function in adhesion might depend on paxillin. Additionally, LPXN regulated cell spreading on CNI but not on fibronectin whereas paxillin knockdown suppressed spreading on both substrates. These results demonstrate that although LPXN and paxillin’s FA targeting and tyrosine phosphorylation are similar, each protein has distinct functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.