ArticleJournal of bacteriology2010
A novel spore protein, ExsM, regulates formation of the exosporium in Bacillus cereus and Bacillus anthracis and affects spore size and shape.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Insights into the Structure and Protein Composition ofInternational journal of molecular sciences · 2022Article
- When Appearance Misleads: The Role of the Entomopathogen Surface in the Relationship with Its Host.Insects · 2020Review
- LysPBC2, a Novel Endolysin Harboring a Bacillus cereus Spore Binding Domain.Applied and environmental microbiology · 2019Article
- Clostridium sordellii outer spore proteins maintain spore structural integrity and promote bacterial clearance from the gastrointestinal tract.PLoS pathogens · 2018Article
- Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis.Scientific reports · 2017Article
- The Regulation of Exosporium-Related Genes in Bacillus thuringiensis.Scientific reports · 2016Article
- The Exosporium Layer of Bacterial Spores: a Connection to the Environment and the Infected Host.Microbiology and molecular biology reviews : MMBR · 2015Review
- Collagen-like glycoprotein BclS is involved in the formation of filamentous structures of the Lysinibacillus sphaericus exosporium.Applied and environmental microbiology · 2014Article
- Bacillus thuringiensis as a surrogate for Bacillus anthracis in aerosol research.World journal of microbiology & biotechnology · 2014Review
- Comparative proteomic analysis of experimental evolution of the Bacillus cereus-Ketogulonicigenium vulgare co-culture.PloS one · 2014Article
- Anthrax SET protein: a potential virulence determinant that epigenetically represses NF-κB activation in infected macrophages.The Journal of biological chemistry · 2013Article
- A genetic approach for the identification of exosporium assembly determinants of Bacillus anthracis.Journal of microbiological methods · 2013Article
- A mimicking-of-DNA-methylation-patterns pipeline for overcoming the restriction barrier of bacteria.PLoS genetics · 2012Article
- Localization and assembly of the novel exosporium protein BetA of Bacillus anthracis.Journal of bacteriology · 2011Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Bacillus cereus spores are assembled with a series of concentric layers that protect them from a wide range of environmental stresses. The outermost layer, or exosporium, is a bag-like structure that interacts with the environment and is composed of more than 20 proteins and glycoproteins. Here, we identified a new spore protein, ExsM, from a beta-mercaptoethanol extract of B. cereus ATCC 4342 spores. Subcellular localization of an ExsM-green fluorescent protein (GFP) protein revealed a dynamic pattern of fluorescence that follows the site of formation of the exosporium around the forespore. Under scanning electron microscopy, exsM null mutant spores were smaller and rounder than wild-type spores, which had an extended exosporium (spore length for the wt, 2.40 +/- 0.56 microm, versus that for the exsM mutant, 1.66 +/- 0.38 microm [P < 0.001]). Thin-section electron microscopy revealed that exsM mutant spores were encased by a double-layer exosporium, both layers of which were composed of a basal layer and a hair-like nap. Mutant exsM spores were more resistant to lysozyme treatment and germinated with higher efficiency than wild-type spores, and they had a delay in outgrowth. Insertional mutagenesis of exsM in Bacillus anthracis DeltaSterne resulted in a partial second exosporium and in smaller spores. In all, these findings suggest that ExsM plays a critical role in the formation of the exosporium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.