ArticleApplied and environmental microbiology2010
Requirement of the type II secretion system for utilization of cellulosic substrates by Cellvibrio japonicus.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- Development of a high-throughput growth assay for bacteria or yeasts using an agar-based insoluble carbon source immobilization method.Microbiology spectrum · 2026Article
- The physiological functions of the Cbp2D and Cbp2E proteins are important for insoluble cellulose-dependent growth inApplied and environmental microbiology · 2025Article
- Current models in bacterial hemicellulase-encoding gene regulation.Applied microbiology and biotechnology · 2024Review
- Green Waste Compost Impacts Microbial Functions Related to Carbohydrate Use and Active Dispersal in Plant Pathogen-Infested Soil.Microbial ecology · 2024Article
- Systematic Analysis of Lysine Acetylation Reveals Diverse Functions in Azorhizobium caulinodans Strain ORS571.Microbiology spectrum · 2023Article
- Cellulose metabolism in halo(natrono)archaea: a comparative genomics study.Frontiers in microbiology · 2023Article
- Long-Term Cellulose Enrichment Selects for Highly Cellulolytic Consortia and Competition for Public Goods.mSystems · 2022Article
- Unifying themes and distinct features of carbon and nitrogen assimilation by polysaccharide-degrading bacteria: a summary of four model systems.Applied microbiology and biotechnology · 2021Review
- Trehalose Degradation by Cellvibrio japonicus Exhibits No Functional Redundancy and Is Solely Dependent on the Tre37A Enzyme.Applied and environmental microbiology · 2020Article
- Mechanisms Underlying the Rhizosphere-To-Rhizoplane Enrichment ofMicroorganisms · 2020Article
- High-throughput screening of environmental polysaccharide-degrading bacteria using biomass containment and complex insoluble substrates.Applied microbiology and biotechnology · 2020Article
- Systems Metabolic Engineering of Escherichia coli Improves Coconversion of Lignocellulose-Derived Sugars.Biotechnology journal · 2019Article
- Systems analysis of the glycoside hydrolase family 18 enzymes fromThe Journal of biological chemistry · 2018Article
- Unique genetic cassettes in aScience advances · 2018Article
- In vitro and in vivo characterization of threeBiotechnology for biofuels · 2018Article
- Comprehensive functional characterization of the glycoside hydrolase family 3 enzymes from Cellvibrio japonicus reveals unique metabolic roles in biomass saccharification.Environmental microbiology · 2017Article
- Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond.Infection and immunity · 2017Review
- Systems analysis in Cellvibrio japonicus resolves predicted redundancy of β-glucosidases and determines essential physiological functions.Molecular microbiology · 2017Article
- Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus.World journal of microbiology & biotechnology · 2016Review
- Structural and Functional Analysis of a Lytic Polysaccharide Monooxygenase Important for Efficient Utilization of Chitin in Cellvibrio japonicus.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellulosic biofuels represent a powerful alternative to petroleum but are currently limited by the inefficiencies of the conversion process. While gram-positive and fungal organisms have been widely explored as sources of cellulases and hemicellulases for biomass degradation, gram-negative organisms have received less experimental attention. We investigated the ability of Cellvibrio japonicus, a recently sequenced gram-negative cellulolytic bacterium, to degrade bioenergy-related feedstocks. Using a newly developed biomass medium, we showed that C. japonicus is able to utilize corn stover and switchgrass as sole sources of carbon and energy for growth. We also developed tools for directed gene disruptions in C. japonicus and used this system to construct a mutant in the gspD gene, which is predicted to encode a component of the type II secretion system. The gspD::pJGG1 mutant displayed a greater-than-2-fold decrease in endoglucanase secretion compared to wild-type C. japonicus. In addition, the mutant strain showed a pronounced growth defect in medium with biomass as a carbon source, yielding 100-fold fewer viable cells than the wild type. To test the potential of C. japonicus to undergo metabolic engineering, we constructed a strain able to produce small amounts of ethanol from biomass. Collectively, these data suggest that C. japonicus is a useful platform for biomass conversion and biofuel production.
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