ArticleApplied and environmental microbiology2010
Specific and rapid enumeration of viable but nonculturable and viable-culturable gram-negative bacteria by using flow cytometry.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 170 citations in OpenAlex.
- Ozonation Eliminates Viable but Not CulturablePathogens (Basel, Switzerland) · 2026Article
- Effects of Nutritional Conditions and Exogenous N-Acyl-Homoserine Lactones on the Resuscitation of Sodium Hypochlorite-Induced DormantMicroorganisms · 2026Article
- Review
- Reducing culture underestimation in urine: flow cytometry-guided validation of low-dose DTT pre-treatment for biofilm dispersal.Microbiology spectrum · 2026Article
- Bioelectrochemical detection of viable but non-culturable bacterial cells on screen-printed carbon electrodes.Biofilm · 2026Article
- Flow Cytometric Screening Reveals Nucleic Acid Dyes Aberrantly Stain Peptidoglycan Sacculi.ACS infectious diseases · 2026Article
- Additive-Free Contact-Electro-Catalysis/Vacuum Ultraviolet System for Rapid Mitigation of Antimicrobial-Resistance-Associated Contaminants in Water.Research (Washington, D.C.) · 2026Article
- Environmental Heterogeneity Drives Ecological Differentiation in Vibrio Populations Across Subtropical Marine Habitats.Environmental microbiology · 2025Article
- Inhibitory Effects of Nisin and Gallium (III) Nitrate Hydrate on Planktonic and Adhered Cells and Implications for the Viable but Non-Culturable State.Microorganisms · 2025Article
- Viable but non-cultivable state in oral microbiota: a critical review of an underexplored microbial survival strategy.Frontiers in cellular and infection microbiology · 2025Review
- Flow cytometry study ofFrontiers in microbiology · 2025Article
- Assessment of safety and in situ antibacterial activity of Weissella cibaria strains isolated from dairy farms in Minas Gerais State, Brazil, for their food application.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Serum susceptibility of Escherichia coli and its association with patient clinical outcomes.PloS one · 2024Article
- Monitoring ofFrontiers in microbiology · 2024Article
- Article
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Changes in salivary microbiota due to gastric cancer resection and its relation to gastric fluid microbiota.Scientific reports · 2023Article
- Detection, Diagnosis, and Preventive Management of the Bacterial Plant PathogenPlants (Basel, Switzerland) · 2023Review
- Role of Resuscitation Promoting Factor-like Protein fromMicroorganisms · 2023Article
- Detection and quantification of viable but non-culturableFrontiers in microbiology · 2023Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An issue of critical concern in microbiology is the ability to detect viable but nonculturable (VBNC) and viable-culturable (VC) cells by methods other than existing approaches. Culture methods are selective and underestimate the real population, and other options (direct viable count and the double-staining method using epifluorescence microscopy and inhibitory substance-influenced molecular methods) are also biased and time-consuming. A rapid approach that reduces selectivity, decreases bias from sample storage and incubation, and reduces assay time is needed. Flow cytometry is a sensitive analytical technique that can rapidly monitor physiological states of bacteria. This report outlines a method to optimize staining protocols and the flow cytometer (FCM) instrument settings for the enumeration of VBNC and VC bacterial cells within 70 min. Experiments were performed using the FCM to quantify VBNC and VC Escherichia coli O157:H7, Pseudomonas aeruginosa, Pseudomonas syringae, and Salmonella enterica serovar Typhimurium cells after staining with different fluorescent probes: SYTO 9, SYTO 13, SYTO 17, SYTO 40, and propidium iodide (PI). The FCM data were compared with those for specific standard nutrient agar to enumerate the number of cells in different states. By comparing results from cultures at late log phase, 1 to 64% of cells were nonculturable, 40 to 98% were culturable, and 0.7 to 4.5% had damaged cell membranes and were therefore theoretically dead. Data obtained using four different gram-negative bacteria exposed to heat and stained with PI also illustrate the usefulness of the approach for the rapid and unbiased detection of dead versus live organisms.
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