Evidence map›Paper›PMID 20543044›Full record

ArticleApplied and environmental microbiology2010

Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.

Alessandro Cascioferro, Francesca Boldrin, Agnese Serafini, Roberta Provvedi, Giorgio Palù, Riccardo Manganelli

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 48 citations in OpenAlex.

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  9. Multiplexed site-specific genome engineering inSynthetic and systems biotechnology · 2022
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  11. Repressible Promoter System to Study Essential Genes in Mycobacteria.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  12. Insertion Mutation ofInfection and drug resistance · 2022
    Article
  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Alessandro CascioferroDepartment of Histology, Microbiology, and Medical Biotechnologies, University of Padova, Via Gabelli 63, 35121 Padova, Italy.
Francesca Boldrin
Agnese Serafini
Roberta Provvedi
Giorgio Palù
Riccardo Manganelli
University of Padua · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetic manipulation of mycobacteria still represents a serious challenge due to the lack of tools and selection markers. In this report, we describe the development of an intrinsically unstable excisable cassette for introduction of unmarked mutations in both Mycobacterium smegmatis and Mycobacterium tuberculosis.

Indexed as

Recombination, GeneticSequence DeletionGenetics, MicrobialMutagenesis, InsertionalMycobacterium smegmatisMycobacterium tuberculosis

Identifiers

PMID20543044
PMCPMC2916470
OpenAlexW2161292266

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.