ArticleNucleic acids research2010
Are two better than one? Analysis of an FtsK/Xer recombination system that uses a single recombinase.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Interplay between the Xer recombination system and the dissemination of antibioresistance in Acinetobacter baumannii.Nucleic acids research · 2025Article
- The effect of site-specific recombinases XerCD on the removal of over-replicated chromosomal DNA through outer membrane vesicles in bacteria.Microbiology spectrum · 2024Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Article
- Bracoviruses recruit host integrases for their integration into caterpillar's genome.PLoS genetics · 2021Article
- Sequence analysis of tyrosine recombinases allows annotation of mobile genetic elements in prokaryotic genomes.Molecular systems biology · 2021Article
- Xer Site Specific Recombination: Double and Single Recombinase Systems.Frontiers in microbiology · 2017Review
- Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending.eLife · 2016Article
- Activation of Xer-recombination at dif: structural basis of the FtsKγ-XerD interaction.Scientific reports · 2016Article
- FtsK translocation permits discrimination between an endogenous and an imported Xer/dif recombination complex.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Conformational transitions during FtsK translocase activation of individual XerCD-dif recombination complexes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The Xer/dif site-specific recombination system of Campylobacter jejuni.Molecular genetics and genomics : MGG · 2013Article
- FtsK actively segregates sister chromosomes in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Co-evolution of segregation guide DNA motifs and the FtsK translocase in bacteria: identification of the atypical Lactococcus lactis KOPS motif.Nucleic acids research · 2012Article
- Xer recombinase and genome integrity in Helicobacter pylori, a pathogen without topoisomerase IV.PloS one · 2012Article
- Activation of XerCD-dif recombination by the FtsK DNA translocase.Nucleic acids research · 2011Article
- Comprehensive prediction of chromosome dimer resolution sites in bacterial genomes.BMC genomics · 2011Article
- Replication termination and chromosome dimer resolution in the archaeon Sulfolobus solfataricus.The EMBO journal · 2011Article
- A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity.PloS one · 2011Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria harbouring circular chromosomes have a Xer site-specific recombination system that resolves chromosome dimers at division. In Escherichia coli, the activity of the XerCD/dif system is controlled and coupled with cell division by the FtsK DNA translocase. Most Xer systems, as XerCD/dif, include two different recombinases. However, some, as the Lactococcus lactis XerS/dif(SL) system, include only one recombinase. We investigated the functional effects of this difference by studying the XerS/dif(SL) system. XerS bound and recombined dif(SL) sites in vitro, both activities displaying asymmetric characteristics. Resolution of chromosome dimers by XerS/dif(SL) required translocation by division septum-borne FtsK. The translocase domain of L. lactis FtsK supported recombination by XerCD/dif, just as E. coli FtsK supports recombination by XerS/dif(SL). Thus, the FtsK-dependent coupling of chromosome segregation with cell division extends to non-rod-shaped bacteria and outside the phylum Proteobacteria. Both the XerCD/dif and XerS/dif(SL) recombination systems require the control activities of the FtsKγ subdomain. However, FtsKγ activates recombination through different mechanisms in these two Xer systems. We show that FtsKγ alone activates XerCD/dif recombination. In contrast, both FtsKγ and the translocation motor are required to activate XerS/dif(SL) recombination. These findings have implications for the mechanisms by which FtsK activates recombination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.