ArticleMolecular cell2010
Systematic analysis of essential genes reveals important regulators of G protein signaling.
Article in Molecular cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 30 citations in OpenAlex.
- Uncovering coordinated pathway interactions through gene co-differential expression in yeast.Nucleic acids research · 2026Article
- Molecular annotation of G protein variants in a neurological disorder.Cell reports · 2023Article
- Catalytic site mutations confer multiple states of G protein activation.Science signaling · 2023Article
- Systematic Identification of Essential Genes Required for Yeast Cell Wall Integrity: Involvement of the RSC Remodelling Complex.Journal of fungi (Basel, Switzerland) · 2022Article
- Review
- A universal allosteric mechanism for G protein activation.Molecular cell · 2021Article
- Regulation of large and small G proteins by ubiquitination.The Journal of biological chemistry · 2019Review
- Negative Feedback Phosphorylation of Gγ Subunit Ste18 and the Ste5 Scaffold Synergistically Regulates MAPK Activation in Yeast.Cell reports · 2018Article
- Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast.The Journal of biological chemistry · 2016Article
- The F-box protein Fbp1 functions in the invasive growth and cell wall integrity mitogen-activated protein kinase (MAPK) pathways in Fusarium oxysporum.Molecular plant pathology · 2016Article
- TTK activates Akt and promotes proliferation and migration of hepatocellular carcinoma cells.Oncotarget · 2015Article
- Structural Analysis of PTM Hotspots (SAPH-ire)--A Quantitative Informatics Method Enabling the Discovery of Novel Regulatory Elements in Protein Families.Molecular & cellular proteomics : MCP · 2015Article
- Why are essential genes essential? - The essentiality ofMicrobial cell (Graz, Austria) · 2015Review
- MAPK feedback encodes a switch and timer for tunable stress adaptation in yeast.Science signaling · 2015Article
- Guanine nucleotide-binding protein (Gα) endocytosis by a cascade of ubiquitin binding domain proteins is required for sustained morphogenesis and proper mating in yeast.The Journal of biological chemistry · 2014Article
- Regulation of yeast G protein signaling by the kinases that activate the AMPK homolog Snf1.Science signaling · 2013Article
- Dynamic ubiquitination of the mitogen-activated protein kinase kinase (MAPKK) Ste7 determines mitogen-activated protein kinase (MAPK) specificity.The Journal of biological chemistry · 2013Article
- HIV-1 Nef impairs heterotrimeric G-protein signaling by targeting Gα(i2) for degradation through ubiquitination.The Journal of biological chemistry · 2012Article
- Genetic and physical interactions between Gα subunits and components of the Gβγ dimer of heterotrimeric G proteins in Neurospora crassa.Eukaryotic cell · 2012Article
- Signal activation and inactivation by the Gα helical domain: a long-neglected partner in G protein signaling.Science signaling · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The yeast pheromone pathway consists of a canonical heterotrimeric G protein and MAP kinase cascade. To identify additional signaling components, we systematically evaluated 870 essential genes using a library of repressible-promoter strains. Quantitative transcription-reporter and MAPK activity assays were used to identify strains that exhibit altered pheromone sensitivity. Of the 92 newly identified essential genes required for proper G protein signaling, those involved with protein degradation were most highly represented. Included in this group are members of the Skp, Cullin, F box (SCF) ubiquitin ligase complex. Further genetic and biochemical analysis reveals that SCF(Cdc4) acts together with the Cdc34 ubiquitin-conjugating enzyme at the level of the G protein; promotes degradation of the G protein alpha subunit, Gpa1, in vivo; and catalyzes Gpa1 ubiquitination in vitro. These insights to the G protein signaling network reveal the essential genome as an untapped resource for identifying new components and regulators of signal transduction pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.