ArticleStructure (London, England : 1993)2010
Unusual target site disruption by the rare-cutting HNH restriction endonuclease PacI.
Article in Structure (London, England : 1993), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Structural and biochemical characterization of yeast Tcd enzymes installing the post-transcriptional modification ct6A in tRNA.Nucleic acids research · 2026Article
- Diversity and functional characterization of HNH endonucleases encoded by lactococcalMicrobial genomics · 2025Article
- An Actively Homing Insertion Element in a Phage Methylase Contains a Hidden HNH Endonuclease.Genes · 2025Article
- Complete genomes of Asgard archaea reveal diverse integrated and mobile genetic elements.Genome research · 2024Article
- DNA Polymerase Diversity Reveals Multiple Incursions of Polintons During Nematode Evolution.Molecular biology and evolution · 2023Article
- Crystal structure and DNA cleavage mechanism of the restriction DNA glycosylase R.CcoLI from Campylobacter coli.Scientific reports · 2021Article
- Structure-Based Deep Mining Reveals First-Time Annotations for 46 Percent of the Dark Annotation Space of the 9,671-Member Superproteome of the Nucleocytoplasmic Large DNA Viruses.Journal of virology · 2020Article
- Distortion of double-stranded DNA structure by the binding of the restriction DNA glycosylase R.PabI.Nucleic acids research · 2020Article
- HK97 gp74 Possesses an α-Helical Insertion in the ββα Fold That Affects Its Metal Binding,Journal of bacteriology · 2020Article
- Crystal structure of the modification-dependent SRA-HNH endonuclease TagI.Nucleic acids research · 2018Article
- Activity and structure of EcoKMcrA.Nucleic acids research · 2018Article
- Systematic classification of the His-Me finger superfamily.Nucleic acids research · 2017Article
- DNA recognition by the SwaI restriction endonuclease involves unusual distortion of an 8 base pair A:T-rich target.Nucleic acids research · 2017Article
- Structural and functional characterization of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease.Scientific reports · 2017Article
- Tetrameric structure of the restriction DNA glycosylase R.PabI in complex with nonspecific double-stranded DNA.Scientific reports · 2016Article
- On the role of steric clashes in methylation control of restriction endonuclease activity.Nucleic acids research · 2016Article
- Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference.Scientific reports · 2015Article
- Structure of 5-hydroxymethylcytosine-specific restriction enzyme, AbaSI, in complex with DNA.Nucleic acids research · 2014Article
- Type II restriction endonucleases--a historical perspective and more.Nucleic acids research · 2014Review
- Elimination of inter-domain interactions increases the cleavage fidelity of the restriction endonuclease DraIII.Protein & cell · 2014Article
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8 authors.
Funding
Abstract
The crystal structure of the rare-cutting HNH restriction endonuclease PacI in complex with its eight-base-pair target recognition sequence 5'-TTAATTAA-3' has been determined to 1.9 A resolution. The enzyme forms an extended homodimer, with each subunit containing two zinc-bound motifs surrounding a betabetaalpha-metal catalytic site. The latter is unusual in that a tyrosine residue likely initiates strand cleavage. PacI dramatically distorts its target sequence from Watson-Crick duplex DNA base pairing, with every base separated from its original partner. Two bases on each strand are unpaired, four are engaged in noncanonical A:A and T:T base pairs, and the remaining two bases are matched with new Watson-Crick partners. This represents a highly unusual DNA binding mechanism for a restriction endonuclease, and implies that initial recognition of the target site might involve significantly different contacts from those visualized in the DNA-bound cocrystal structures.
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