ArticleStructure (London, England : 1993)2010
Three-dimensional structure of TspO by electron cryomicroscopy of helical crystals.
Article in Structure (London, England : 1993), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 103 citations in OpenAlex.
- Neurospecific Molecules Measured in Periphery in Humans: How Do They Correlate with the Brain Levels? A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Imaging neuroinflammation with TSPO: A new perspective on the cellular sources and subcellular localization.Pharmacology & therapeutics · 2022Review
- Comparative Assessment of TSPO Modulators on Electroencephalogram Activity and Exploratory Behavior.Frontiers in pharmacology · 2022Article
- Cellular sources of TSPO expression in healthy and diseased brain.European journal of nuclear medicine and molecular imaging · 2021Review
- TSPO protein binding partners in bacteria, animals, and plants.Journal of bioenergetics and biomembranes · 2021Review
- Insight into the Structural Features of TSPO: Implications for Drug Development.Trends in pharmacological sciences · 2020Review
- Be Cautious with Crystal Structures of Membrane Proteins or Complexes Prepared in Detergents.Crystals · 2020Article
- VDAC1 and the TSPO: Expression, Interactions, and Associated Functions in Health and Disease States.International journal of molecular sciences · 2019Review
- A unified structural model of the mammalian translocator protein (TSPO).Journal of biomolecular NMR · 2019Article
- PET Evaluation of Microglial Activation in Non-neurodegenerative Brain Diseases.Current neurology and neuroscience reports · 2019Review
- Characterization of the High-Affinity Drug Ligand Binding Site of Mouse Recombinant TSPO.International journal of molecular sciences · 2019Article
- TSPO: An Evolutionarily Conserved Protein with Elusive Functions.International journal of molecular sciences · 2018Review
- Challenges and approaches to understand cholesterol-binding impact on membrane protein function: an NMR view.Cellular and molecular life sciences : CMLS · 2018Review
- In vivo PET imaging of neuroinflammation in Alzheimer's disease.Journal of neural transmission (Vienna, Austria : 1996) · 2018Review
- Applications of sequence coevolution in membrane protein biochemistry.Biochimica et biophysica acta. Biomembranes · 2018Review
- Translocator protein (18 kDa): an update on its function in steroidogenesis.Journal of neuroendocrinology · 2018Review
- Expression and purification of the mammalian translocator protein for structural studies.PloS one · 2018Article
- The Mitochondrial Translocator Protein and the Emerging Link Between Oxidative Stress and Arrhythmias in the Diabetic Heart.Frontiers in physiology · 2018Review
- Reconceptualization of translocator protein as a biomarker of neuroinflammation in psychiatry.Molecular psychiatry · 2018Review
- A Farnesylated Coxiella burnetii Effector Forms a Multimeric Complex at the Mitochondrial Outer Membrane during Infection.Infection and immunity · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The 18 kDa TSPO protein is a polytopic mitochondrial outer membrane protein involved in a wide range of physiological functions and pathologies, including neurodegeneration and cancer. The pharmacology of TSPO has been extensively studied, but little is known about its biochemistry, oligomeric state, and structure. We have expressed, purified, and characterized a homologous protein, TspO from Rhodobacter sphaeroides, and reconstituted it as helical crystals. Using electron cryomicroscopy and single-particle helical reconstruction, we have determined a three-dimensional structure of TspO at 10 A resolution. The structure suggests that monomeric TspO comprises five transmembrane alpha helices that form a homodimer, which is consistent with the dimeric state observed in detergent solution. Furthermore, the arrangement of transmembrane domains of individual TspO subunits indicates a possibility of two substrate translocation pathways per dimer. The structure provides the first insight into the molecular architecture of TSPO/PBR protein family that will serve as a framework for future studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.