ArticleJournal of immunological methods2010
Stromal cell-free conditions favorable for human B lymphopoiesis in culture.
Article in Journal of immunological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Electroporation for High-Efficiency Delivery of CRISPR to Hematopoietic Cells.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Article
- ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy development.Cell reports. Medicine · 2026Article
- B-Cell Differentiation of Human Hematopoietic Progenitors Is Efficiently Supported by Wharton Jelly-Derived Mesenchymal Stem Cells.European journal of immunology · 2026Article
- Hematopoietic stem cell gene editing rescues B-cell development in X-linked agammaglobulinemia.The Journal of allergy and clinical immunology · 2024Article
- Impact of 2 Gy γ-irradiation on the hallmark characteristics of human bone marrow-derived MSCs.International journal of hematology · 2021Article
- TLR engagement induces ARID3a in human blood hematopoietic progenitors and modulates IFNα production.Cellular immunology · 2020Article
- Improved human hematopoietic reconstitution in HepaRG co-transplanted humanized NSG mice.BMB reports · 2020Article
- ARID3a expression in human hematopoietic stem cells is associated with distinct gene patterns in aged individuals.Immunity & ageing : I & A · 2020Article
- Effects of autologous stromal cells and cytokines on differentiation of equine bone marrow-derived progenitor cells.American journal of veterinary research · 2017Article
- Aryl hydrocarbon receptor activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin impairs human B lymphopoiesis.Toxicology · 2017Article
- BTK gene targeting by homologous recombination using a helper-dependent adenovirus/adeno-associated virus hybrid vector.Gene therapy · 2016Article
- The Transcription Factor ARID3a Is Important for In Vitro Differentiation of Human Hematopoietic Progenitors.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Pro-inflammatory-Related Loss of CXCL12 Niche Promotes Acute Lymphoblastic Leukemic Progression at the Expense of Normal Lymphopoiesis.Frontiers in immunology · 2016Article
- Differential expression of the transcription factor ARID3a in lupus patient hematopoietic progenitor cells.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Early B lymphocyte development: Similarities and differences in human and mouse.World journal of stem cells · 2014Review
- The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.Blood · 2012Article
- The density of CD10 corresponds to commitment and progression in the human B lymphoid lineage.PloS one · 2010Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Progress has been slow in defining molecular requirements for human B lymphopoiesis in part because of differences from experimental animals and also because of the lack of culture conditions that efficiently support the process. We recently found that human CD10+ lymphocytes were produced when CD34+ hematopoietic stem and progenitor cells were cultured in contact with human mesenchymal stem cells (hMSC). Further investigation revealed that it occurred even when progenitors were separated from hMSC by membrane filters. Experiments with neutralizing antibodies suggested that important heat labile factors produced by hMSC are unlikely to be IL-7, TSLP, CXCL12 or hemokinin-1. Further manipulation of culture conditions revealed that optimal lymphopoiesis required careful selection of fetal calf serum lots, maintenance of high cell densities, as well as recombinant cytokines (SCF, FL and G-CSF). G-CSF was particularly important when adult bone marrow rather than umbilical cord blood derived CD34+ cells were used to initiate the cultures. These improved methods should facilitate identification of molecules that can be used to speed regeneration of the humoral immune system following chemotherapy and might suggest ways to inhibit growth of B lineage malignancies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.