ArticleBMC microbiology2010
In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae, African strain MAI1.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Proteomic and Transcriptomic Analyses Provide Novel Insights into the Crucial Roles of Host-Induced Carbohydrate Metabolism Enzymes in Xanthomonas oryzae pv. oryzae Virulence and Rice-Xoo Interaction.Rice (New York, N.Y.) · 2021Article
- Identification and expression of genes in response to cassava bacterial blight infection.Journal of applied genetics · 2018Article
- Transcriptome analysis of the Dickeya dadantii PecS regulon during the early stages of interaction with Arabidopsis thaliana.Molecular plant pathology · 2018Article
- Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae.PloS one · 2018Article
- Global Transcriptome Profiling ofThe plant pathology journal · 2017Article
- Analysis of thePeerJ · 2017Article
- Article
- Role of DetR in defence is critical for virulence of Xanthomonas oryzae pv. oryzae.Molecular plant pathology · 2016Article
- Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis.PeerJ · 2016Article
- An optimized method for the extraction of bacterial mRNA from plant roots infected with Escherichia coli O157:H7.Frontiers in microbiology · 2014Article
- Genome mining reveals the genus Xanthomonas to be a promising reservoir for new bioactive non-ribosomally synthesized peptides.BMC genomics · 2013Article
- Multilocus sequence analysis and type III effector repertoire mining provide new insights into the evolutionary history and virulence of Xanthomonas oryzae.Molecular plant pathology · 2012Article
- Pathogenomics of Xanthomonas: understanding bacterium-plant interactions.Nature reviews. Microbiology · 2011Review
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7 authors.
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Abstract
backgroundBacterial leaf blight causes significant yield losses in rice crops throughout Asia and Africa. Although both the Asian and African strains of the pathogen, Xanthomonas oryzae pv. oryzae (Xoo), induce similar symptoms, they are nevertheless genetically different, with the African strains being more closely related to the Asian X. oryzae pv. oryzicola (Xoc).
resultsChanges in gene expression of the African Xoo strain MAI1 in the susceptible rice cultivar Nipponbare were profiled, using an SSH Xoo DNA microarray. Microarray hybridization was performed comparing bacteria recovered from plant tissues at 1, 3, and 6 days after inoculation (dai) with bacteria grown in vitro. A total of 710 bacterial genes were found to be differentially expressed, with 407 up-regulated and 303 down-regulated. Expression profiling indicated that less than 20% of the 710 bacterial transcripts were induced in the first 24 h after inoculation, whereas 63% were differentially expressed at 6 dai. The 710 differentially expressed genes were one-end sequenced. 535 sequences were obtained from which 147 non-redundant sequences were identified. Differentially expressed genes were related to metabolism, secretion and transport, pathogen adherence to plant tissues, plant cell-wall degradation, IS elements, and virulence. In addition, various other genes encoding proteins with unknown function or showing no similarity to other proteins were also induced. The Xoo MAI1 non-redundant set of sequences was compared against several X. oryzae genomes, revealing a specific group of genes that was present only in MAI1. Numerous IS elements were also found to be differentially expressed. Quantitative real-time PCR confirmed 86% of the identified profile on a set of 14 genes selected according to the microarray analysis.
conclusionsThis is the first report to compare the expression of Xoo genes in planta across different time points during infection. This work shows that as-yet-unidentified and potentially new virulence factors are appearing in an emerging African pathogen. It also confirms that African Xoo strains do differ from their Asian counterparts, even at the transcriptional level.
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