ArticlePloS one2010
Parthenogenic blastocysts derived from cumulus-free in vitro matured human oocytes.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Trial
- Artificial activation of clinically failed human oocytes enables euploid blastocyst formation.Journal of assisted reproduction and genetics · 2026Article
- Successful Clinical Outcome After a Modified Protocol of Rescue In Vitro Maturation of Oocytes: A Case Series Study.Cureus · 2025Article
- Impact of Ovarian Factor Mediums on the Apoptotic Gene Expression and Embryo Quality Derived From Vitrified Immature Human Oocytes.Journal of obstetrics and gynaecology of India · 2023Article
- Effect of ovarian growth factors on ultra-structural maturation in frozen human immature oocytes after in vitro maturation: a comparative study.Reproductive health · 2022Article
- Differential transcriptional profiles of human cumulus granulosa cells in patients with diminished ovarian reserve.Archives of gynecology and obstetrics · 2022Article
- Effects of microvibration stimulation on developmental potential of discarded germinal vesicle oocytes of human.Frontiers in endocrinology · 2022Article
- Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence-A Transcriptomic Study.International journal of molecular sciences · 2019Article
- Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres.Human molecular genetics · 2014Article
- In vitro maturation may prevent the cancellation of in vitro fertilization cycles in poor responder patients: A case report.Journal of the Turkish German Gynecological Association · 2013Article
- Variable imprinting of the MEST gene in human preimplantation embryos.European journal of human genetics : EJHG · 2013Article
- Ovarian adipocytokines are associated with early in vitro human embryo development independent of the action of ovarian insulin.Journal of assisted reproduction and genetics · 2012Article
- In-vitro maturation of human oocytes: before or after vitrification?Journal of assisted reproduction and genetics · 2012Article
- Altered cell cycle gene expression and apoptosis in post-implantation dog parthenotes.PloS one · 2012Article
- Finding the niche for human somatic cell nuclear transfer.Nature biotechnology · 2011Article
- Germline competency of parthenogenetic embryonic stem cells from immature oocytes of adult mouse ovary.Human molecular genetics · 2011Article
- Denudation and sperm injection interval timing: impact on outcome of intracytoplasmic sperm injection.International journal of women's health · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundApproximately 20% of oocytes are classified as immature and discarded following intracytoplasmic sperm injection (ICSI) procedures. These oocytes are obtained from gonadotropin-stimulated patients, and are routinely removed from the cumulus cells which normally would mature the oocytes. Given the ready access to these human oocytes, they represent a potential resource for both clinical and basic science application. However culture conditions for the maturation of cumulus-free oocytes have not been optimized. We aimed to improve maturation conditions for cumulus-free oocytes via culture with ovarian paracrine/autocrine factors identified by single cell analysis. METHODOLOGY/PRINCIPAL FINDING: Immature human oocytes were matured in vitro via supplementation with ovarian paracrine/autocrine factors that were selected based on expression of ligands in the cumulus cells and their corresponding receptors in oocytes. Matured oocytes were artificially activated to assess developmental competence. Gene expression profiles of parthenotes were compared to IVF/ICSI embryos at morula and blastocyst stages. Following incubation in medium supplemented with ovarian factors (BDNF, IGF-I, estradiol, GDNF, FGF2 and leptin), a greater percentage of oocytes demonstrated nuclear maturation and subsequently, underwent parthenogenesis relative to control. Similarly, cytoplasmic maturation was also improved as indicated by development to blastocyst stage. Parthenogenic blastocysts exhibited mRNA expression profiles similar to those of blastocysts obtained after IVF/ICSI with the exception for MKLP2 and PEG1. CONCLUSIONS/SIGNIFICANCE: Human cumulus-free oocytes from hormone-stimulated cycles are capable of developing to blastocysts when cultured with ovarian factor supplementation. Our improved IVM culture conditions may be used for obtaining mature oocytes for clinical purposes and/or for derivation of embryonic stem cells following parthenogenesis or nuclear transfer.
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