ArticleOrganogenesis2009
Imaging growth of neurites in conditioned hydrogel by coherent anti-stokes raman scattering microscopy.
Article in Organogenesis, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- In vivo non-invasive monitoring of tissue development in 3D printed subcutaneous bone scaffolds using fibre-optic Raman spectroscopy.Biomaterials and biosystems · 2022Article
- Secondary ion mass spectrometry and Raman spectroscopy for tissue engineering applications.Current opinion in biotechnology · 2015Review
- Third harmonic generation microscopy of a mouse retina.Molecular vision · 2015Article
- Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine.Journal of the Royal Society, Interface · 2013Review
- Imaging the intact mouse cornea using coherent anti-stokes Raman scattering (CARS).Investigative ophthalmology & visual science · 2013Article
- Coherent anti-stokes Raman scattering (CARS) microscopy: a novel technique for imaging the retina.Investigative ophthalmology & visual science · 2013Article
- Single cell optical imaging and spectroscopy.Chemical reviews · 2013Review
- Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy.Current opinion in genetics & development · 2011Review
- Multimodal Nonlinear Optical Microscopy.Laser & photonics reviews · 2011Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cultured DRGs in different gel scaffolds were analyzed using CA RS microscopy to determine its possible use as a label-free imaging option for tracking cellular growth in a gel scaffold. This study demonstrates for the first time the applicability of CA RS microscopy to the imaging of live neuronal cells in GAG hydrogels. By tuning the laser beating frequency, omega(p)-omega(s), to match the vibration of C-H bonds in the cell membrane, the CA RS signal yields detailed, high-quality images of neurites with single membrane detection sensitivity. The results demonstrate that CA RS imaging allows monitoring of cellular growth in a tissue scaffold over time, with a contrast that shows comparable cellular structures to those obtained using standard fluorescent staining techniques. These findings show the potential of CARS microscopy to assist in the understanding of organogenesis processes in a tissue scaffold.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.