ArticleNature protocols2010
A fluorescence-based in vitro assay for investigating early endosome dynamics.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- The assembly of RAB22A/TMEM33/RTN4 initiates a secretory ER-phagy pathway.Cell discovery · 2025Article
- Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic.Nature communications · 2024Article
- Temporal changes in plasma membrane lipid content induce endocytosis to regulate developmental epithelial-to-mesenchymal transition.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- PTPN9-mediated dephosphorylation of VTI1B promotes ATG16L1 precursor fusion and autophagosome formation.Autophagy · 2021Article
- Article
- Cdc48/VCP and Endocytosis Regulate TDP-43 and FUS Toxicity and Turnover.Molecular and cellular biology · 2020Article
- Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.Scientific reports · 2019Article
- Activity-dependent bulk endocytosis proteome reveals a key presynaptic role for the monomeric GTPase Rab11.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17.The Journal of cell biology · 2018Article
- Cytosolic Uptake of Large Monofunctionalized Dextrans.Bioconjugate chemistry · 2018Article
- GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.Developmental cell · 2018Article
- Probing and manipulating intracellular membrane traffic by microinjection of artificial vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- HCV-induced autophagosomes are generated via homotypic fusion of phagophores that mediate HCV RNA replication.PLoS pathogens · 2017Article
- Septins promote macropinosome maturation and traffic to the lysosome by facilitating membrane fusion.The Journal of cell biology · 2016Article
- Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.Cell research · 2016Article
- Article
- Methods to analyze SNARE-dependent vesicular fusion events that regulate autophagosome biogenesis.Methods (San Diego, Calif.) · 2015Article
- A novel size-based sorting mechanism of pinocytic luminal cargoes in microglia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- RAB-5 and RAB-10 cooperate to regulate neuropeptide release in Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early endosomes receive material from the plasma membrane by fusion with endocytotic vesicles. This material is sorted within endosomes and directed to subdomains at which carrier vesicles bud. These vesicles are then transported toward different cellular destinations. In this article, we describe a protocol for the cell-free reconstitution of endosome docking/fusion and sorting/budding, which is based on labeling of endosomes by endocytotic uptake with fluorescent cargoes. The protocol includes (i) the preparation of fluorescently labeled endosomes, (ii) assays for docking/fusion and for sorting/budding in vitro and (iii) imaging of the reaction mix by fluorescence microscopy to quantify docking, fusion, cargo sorting and budding using counting of single organelles. Production of endosome stocks requires approximately 1 d. The in vitro reactions can then be performed separately (approximately 1 d) and are conveniently carried out with multiple samples in parallel. The assay can be adapted for studying the dynamics of organelles other than endosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.