ArticleNature protocols2010
Leishmania parasite detection and quantification using PCR-ELISA.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Strategy for Improving the Diagnostic Efficacy of Bovine Tuberculosis Using a Novel PCRVeterinary research communications · 2026Article
- Non-additive strong gene interactions cause striking differences in organ pathology and cytokine response in Leishmaniasis.Frontiers in immunology · 2025Article
- Molecular Diagnosis of Human Cutaneous Leishmaniasis and Identification of the Causative Leishmania Species in Iran: A Narrative ReviewIranian biomedical journal · 2024Review
- Review
- Natural history of the parasiteParasitology · 2023Article
- Functionally distinct regions of the locusFrontiers in immunology · 2023Article
- Novel Loci Controlling Parasite Load in Organs of Mice Infected WithFrontiers in immunology · 2019Article
- Sensitive detection of the ISRSC advances · 2018Article
- Molecular Diagnosis of Visceral Leishmaniasis.Molecular diagnosis & therapy · 2018Review
- Genetic Regulation of Guanylate-Binding Proteins 2b and 5 during Leishmaniasis in Mice.Frontiers in immunology · 2018Article
- A real-time PCR assay for quantification of parasite burden in murine models of leishmaniasis.PeerJ · 2018Article
- Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection byJournal of tropical medicine · 2017Article
- Gene-specific sex effects on eosinophil infiltration in leishmaniasis.Biology of sex differences · 2016Article
- Article
- Application of PCR-ELISA in molecular diagnosis.BioMed research international · 2014Review
- Detection of Leishmania infantum and identification of blood meals in Phlebotomus perniciosus from a focus of human leishmaniasis in Madrid, Spain.Parasitology research · 2013Article
- Mapping the genes for susceptibility and response to Leishmania tropica in mouse.PLoS neglected tropical diseases · 2013Article
- Genetics of host response to Leishmania tropica in mice - different control of skin pathology, chemokine reaction, and invasion into spleen and liver.PLoS neglected tropical diseases · 2012Article
- Immunization against Leishmania major infection using LACK- and IL-12-expressing Lactococcus lactis induces delay in footpad swelling.PloS one · 2012Article
- Detection of E. coli O157:H7 and Shigella dysenteriae toxins in clinical samples by PCR-ELISA.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol describes an improved and optimized PCR-ELISA method for detection and quantification of Leishmania parasites in host tissues. Unlike other DNA-based assays, this method uses digoxigenin- and biotin-labeled primers. This eliminates the need for a separate step of hybridization of the PCR product with labeled probes. The PCR product is detected using sandwich ELISA with antidigoxigenin-detecting antibodies. Primers are complementary to the kinetoplast minicircle conserved region of parasite DNA, allowing the detection of several Leishmania species. For measurement of a wide range of parasite concentrations, +/-25 cycles were optimal. The sensitivity of this technique is 0.3 fg of parasite DNA per reaction in 40-cycle PCR-ELISA, corresponding to 0.004 parasites. DNA preparation by a standard TRI reagent procedure takes about 4 h. When DNA is prepared, a single person can test a large number of samples (at least 150) in a maximum of 7 h. This method might also be suitable for detecting and quantifying other pathogens, especially for detecting small differences in pathogen numbers.
Indexed as
Identifiers
20539283What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.