ArticleNature protocols2010
Immobilization of growth factors on solid supports for the modulation of stem cell fate.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 52 citations in OpenAlex.
- Spatial Stem Cell Fate Engineering via Facile Morphogen Localization.Advanced healthcare materials · 2021Article
- Stimuli-Responsive Delivery of Growth Factors for Tissue Engineering.Advanced healthcare materials · 2020Review
- A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion.Matrix biology plus · 2019Article
- Hydrolytically Labile Linkers Regulate Release and Activity of Human Bone Morphogenetic Protein-6.Langmuir : the ACS journal of surfaces and colloids · 2018Article
- Prolonged survival of transplanted stem cells after ischaemic injury via the slow release of pro-survival peptides from a collagen matrix.Nature biomedical engineering · 2018Article
- Matrix Metalloproteinase Responsive Delivery of Myostatin Inhibitors.Pharmaceutical research · 2017Article
- Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications.Journal of visualized experiments : JoVE · 2016Article
- Investigation of the changes of biophysical/mechanical characteristics of differentiating preosteoblasts in vitro.Biomaterials research · 2015Article
- 3D niche microarrays for systems-level analyses of cell fate.Nature communications · 2014Article
- Controlled release strategies for bone, cartilage, and osteochondral engineering--Part II: challenges on the evolution from single to multiple bioactive factor delivery.Tissue engineering. Part B, Reviews · 2013Review
- MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state.PloS one · 2013Article
- Control of stem cell fate and function by engineering physical microenvironments.Integrative biology : quantitative biosciences from nano to macro · 2012Review
- Engineering airway epithelium.Journal of biomedicine & biotechnology · 2012Review
- Optimizing stem cell culture.Journal of cellular biochemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Surface- and matrix-bound signals modulate stem cell fate in vivo and in vitro. This protocol enables the immobilization of a wide range of biomolecules that contain primary amino groups to different types of solid carriers, including glass substrates and standard polystyrene well plates. We describe how thin polymer coatings of poly(octadecene-alt-maleic anhydride) can be used to covalently attach growth factors directly, or through poly(ethylene glycol) spacers, to solid supports at defined concentrations. Surface-immobilized growth factors can be presented over a wide range of concentrations (5-150 ng cm(-2)), as we have previously shown for leukemia inhibitory factor and stem cell factor. Cell activation can be achieved in the presence of adhesion-promoting extracellular matrix proteins. Depending on the methods used, the overall procedure takes 1.5-3 d. In general, the approach can be used to investigate the effect of defined amounts of immobilized growth factors on stem cells and on the maintenance, growth and differentiation of other cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.