ArticleNature protocols2010
High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01378013 (Metabolic Phenotyping and Systems Biology in Surgery), which is not on this map. Cited by 130 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Metabolic Phenotyping and Systems Biology in Surgery
Who cites it
130 citing papers in PubMed.
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- Fast three-dimensional NMR spectroscopy reconstruction based on Hankel matrix with two-directional sliding windows.Magnetic resonance letters · 2026Article
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- Lipidomic alterations in oysters caused by environmentally relevant exposure to microplastics and estrogenic endocrine disrupting chemicals.Ecotoxicology (London, England) · 2026Article
- Live Metabolomics with NMR.Analytical chemistry · 2026Review
- NMR metabolomics reveals metabolic alterations in a novel mouse model of neurodegeneration.Frontiers in neuroscience · 2026Article
- Osmolytes vs. Anabolic Reserves: Contrasting Gonadal Metabolomes in Two Sympatric Mediterranean Sea Urchins.Metabolites · 2025Article
- Role of metabolomic changes in diagnosis and assessment of treatment response in acute myeloid leukemia.Scientific reports · 2025Article
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- Navigating the challenges of engineering composite specialized metabolite pathways in plants.The Plant journal : for cell and molecular biology · 2025Review
- MYC-dependent upregulation of the de novo serine and glycine synthesis pathway is a targetable metabolic vulnerability in group 3 medulloblastoma.Neuro-oncology · 2025Article
- Functional metabolomics: unlocking the role of small molecular metabolites.Frontiers in molecular biosciences · 2025Review
- Comparative lipidomic and metabolomic profiling of mdx and severe mdx-apolipoprotein e-null mice.Skeletal muscle · 2024Article
- High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy of Paired Clinical Liver Tissue Samples from Hepatocellular Cancer and Surrounding Region.International journal of molecular sciences · 2024Article
- NMR spectroscopy for metabolomics in the living system: recent progress and future challenges.Analytical and bioanalytical chemistry · 2024Review
- Maternal exposure to polystyrene nanoplastics alters fetal brain metabolism in mice.Metabolomics : Official journal of the Metabolomic Society · 2023Article
- To metabolomics and beyond: a technological portfolio to investigate cancer metabolism.Signal transduction and targeted therapy · 2023Review
- The Potential Role of Metabolomics in Drug-Induced Liver Injury (DILI) Assessment.Metabolites · 2022Review
70 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic profiling, metabolomic and metabonomic studies require robust study protocols for any large-scale comparisons and evaluations. Detailed methods for solution-state NMR spectroscopy have been summarized in an earlier protocol. This protocol details the analysis of intact tissue samples by means of high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy and we provide a detailed description of sample collection, preparation and analysis. Described here are (1)H NMR spectroscopic techniques such as the standard one-dimensional, relaxation-edited, diffusion-edited and two-dimensional J-resolved pulse experiments, as well as one-dimensional (31)P NMR spectroscopy. These are used to monitor different groups of metabolites, e.g., sugars, amino acids and osmolytes as well as larger molecules such as lipids, non-invasively. Through the use of NMR-based diffusion coefficient and relaxation times measurements, information on molecular compartmentation and mobility can be gleaned. The NMR methods are often combined with statistical analysis for further metabonomics analysis and biomarker identification. The standard acquisition time per sample is 8-10 min for a simple one-dimensional (1)H NMR spectrum, giving access to metabolite information while retaining tissue integrity and hence allowing direct comparison with histopathology and MRI/MRS findings or the evaluation together with biofluid metabolic-profiling data.
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Registered trials
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