ArticleProceedings of the National Academy of Sciences of the United States of America2010
Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Divergent Rickettsia species exhibit distinct mechanisms of actin-based motility.The Journal of cell biology · 2026Article
- Structural and functional dissection of the WH2/DAD motif of INF2, a formin linked to human inherited degenerative disorders.The FEBS journal · 2026Article
- Interaction of cardiac leiomodin with the native cardiac thin filament.PLoS biology · 2025Article
- Actomyosin organelle functions of SPIRE actin nucleators precede animal evolution.Communications biology · 2024Article
- Structural insights into actin isoforms.eLife · 2023Article
- The actin nucleator Spir-1 is a virus restriction factor that promotes innate immune signalling.PLoS pathogens · 2022Article
- Zero-mode waveguides visualize the first steps during gelsolin-mediated actin filament formation.Biophysical journal · 2022Article
- Insights into Actin Polymerization and Nucleation Using a Coarse-Grained Model.Biophysical journal · 2020Article
- Biochemical Activities of the Wiskott-Aldrich Syndrome Homology Region 2 Domains of Sarcomere Length Short (SALS) Protein.The Journal of biological chemistry · 2016Article
- Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif.Structure (London, England : 1993) · 2015Article
- Filament assembly by Spire: key residues and concerted actin binding.Journal of molecular biology · 2015Article
- Article
- Structural basis of actin filament nucleation by tandem W domains.Cell reports · 2013Article
- In vitro studies of actin filament and network dynamics.Current opinion in cell biology · 2013Review
- Ablation of cyclase-associated protein 2 (CAP2) leads to cardiomyopathy.Cellular and molecular life sciences : CMLS · 2013Article
- Multiple forms of Spire-actin complexes and their functional consequences.The Journal of biological chemistry · 2012Article
- How a single residue in individual β-thymosin/WH2 domains controls their functions in actin assembly.The EMBO journal · 2012Article
- Three-dimensional reconstructions of Arp2/3 complex with bound nucleation promoting factors.The EMBO journal · 2012Article
- Connecdenn 3/DENND1C binds actin linking Rab35 activation to the actin cytoskeleton.Molecular biology of the cell · 2012Article
- Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
Three classes of proteins are known to nucleate new filaments: the Arp2/3 complex, formins, and the third group of proteins that contain ca. 25 amino acid long actin-binding Wiskott-Aldrich syndrome protein homology 2 domains, called the WH2 repeats. Crystal structures of the complexes between the actin-binding WH2 repeats of the Spire protein and actin were determined for the Spire single WH2 domain D, the double (SpirCD), triple (SpirBCD), quadruple (SpirABCD) domains, and an artificial Spire WH2 construct comprising three identical D repeats (SpirDDD). SpirCD represents the minimal functional core of Spire that can nucleate actin filaments. Packing in the crystals of the actin complexes with SpirCD, SpirBCD, SpirABCD, and SpirDDD shows the presence of two types of assemblies, "side-to-side" and "straight-longitudinal," which can serve as actin filament nuclei. The principal feature of these structures is their loose, open conformations, in which the sides of actins that normally constitute the inner interface core of a filament are flipped inside out. These Spire structures are distant from those seen in the filamentous nuclei of Arp2/3, formins, and in the F-actin filament.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.