ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2010
Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Genetic Variants Influencing Taste Perception and Food Preferences: Current Evidence.Nutrients · 2026Review
- Sensing Underwater: Diversifying Selection, Convergent Evolution and Inactivation in Sensory Receptors' Genes of Aquatic Mammals.Journal of molecular evolution · 2026Article
- Epitope Tagging with Genome Editing in Mice Reveals That the Proton Channel OTOP1 Is Apically Localized and Not Restricted to Type III "Sour" Taste Receptor Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Characterization of host factors associated with the internal ribosomal entry sites of foot-and-mouth disease and classical swine fever viruses.Scientific reports · 2022Article
- Adaptive selection drives TRPP3 loss-of-function in an Ethiopian population.Scientific reports · 2020Article
- High-throughput inference of pairwise coalescence times identifies signals of selection and enriched disease heritability.Nature genetics · 2018Article
- Extracellular Loops Are Essential for the Assembly and Function of Polycystin Receptor-Ion Channel Complexes.The Journal of biological chemistry · 2017Article
- Regulation of TRPP3 Channel Function by N-terminal Domain Palmitoylation and Phosphorylation.The Journal of biological chemistry · 2016Article
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- Acid-induced off-response of PKD2L1 channel in Xenopus oocytes and its regulation by Ca(2.).Scientific reports · 2015Article
- Chemosensory receptor specificity and regulation.Annual review of neuroscience · 2015Review
- A novel PKD2L1 C-terminal domain critical for trimerization and channel function.Scientific reports · 2015Article
- Structural biology of TRP channels.Handbook of experimental pharmacology · 2014Review
- Properties of subependymal cerebrospinal fluid contacting neurones in the dorsal vagal complex of the mouse brainstem.The Journal of physiology · 2012Article
- The response of PKD1L3/PKD2L1 to acid stimuli is inhibited by capsaicin and its pungent analogs.The FEBS journal · 2012Article
- Receptor for activated C kinase 1 (RACK1) inhibits function of transient receptor potential (TRP)-type channel Pkd2L1 through physical interaction.The Journal of biological chemistry · 2012Article
- Molecular mechanism of the assembly of an acid-sensing receptor ion channel complex.Nature communications · 2012Article
- Genetic tracing of the gustatory neural pathway originating from Pkd1l3-expressing type III taste cells in circumvallate and foliate papillae.Journal of neurochemistry · 2011Article
- Bimodal effect of alkalization on the polycystin transient receptor potential channel, PKD2L1.Pflugers Archiv : European journal of physiology · 2011Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
The polycystic kidney disease 1-like 3 (PKD1L3) and polycystic kidney disease 2-like 1 (PKD2L1) proteins have been proposed to form heteromers that function as sour taste receptors in mammals. Here, we show that PKD1L3 and PKD2L1 interact through their transmembrane domains, and not through the coiled-coil domain, by coimmunoprecipitation experiments using a series of deletion mutants. Deletion mutants lacking the critical interaction region were not transported to the cell surface and remained in the cytoplasm, whereas PKD1L3 and PKD2L1 proteins were expressed at the cell surface when both are transfected. Calcium imaging analysis revealed that neither the coiled-coil domain nor the EF-hand domain located in the C-terminal cytoplasmic tail of PKD2L1 was required for response on stimulation with an acidic solution. Finally, PKD2L1 did not localize to the taste pore but was distributed throughout the cytoplasm in taste cells of circumvallate and foliate papillae in PKD1L3(-/-) mice, whereas it localized to the taste pore in wild-type mice. Collectively, these results suggest that the interaction between PKD1L3 and PKD2L1 through their transmembrane domains is essential for proper trafficking of the channels to the cell surface in taste cells of circumvallate and foliate papillae and in cultured cells.
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