ArticleJournal of molecular biology2010
Architecture of the Tn7 posttransposition complex: an elaborate nucleoprotein structure.
Article in Journal of molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Natural and Engineered Guide RNA-Directed Transposition with CRISPR-Associated Tn7-Like Transposons.Annual review of biochemistry · 2024Review
- Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases.Nature biotechnology · 2024Article
- Targeted DNA integration in human cells without double-strand breaks using CRISPR RNA-guided transposases.bioRxiv : the preprint server for biology · 2023Article
- Structure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon.Nature communications · 2022Article
- Article
- Structural basis of transposon end recognition explains central features of Tn7 transposition systems.Molecular cell · 2022Article
- Structural basis for DNA targeting by the Tn7 transposon.Nature structural & molecular biology · 2022Article
- Article
- Recruitment of CRISPR-Cas systems by Tn7-like transposons.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Mechanisms of DNA Transposition.Microbiology spectrum · 2015Review
- Controlling DNA degradation from a distance: a new role for the Mu transposition enhancer.Molecular microbiology · 2014Article
- The Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Direct interaction between the TnsA and TnsB subunits controls the heteromeric Tn7 transposase.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The emerging diversity of transpososome architectures.Quarterly reviews of biophysics · 2012Review
- Moving DNA around: DNA transposition and retroviral integration.Current opinion in structural biology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Four transposition proteins encoded by the bacterial transposon Tn7, TnsA, TnsB, TnsC, and TnsD, mediate its site- and orientation-specific insertion into the chromosomal site attTn7. To establish which Tns proteins are actually present in the transpososome that executes DNA breakage and joining, we have determined the proteins present in the nucleoprotein product of transposition, the posttransposition complex (PTC), using fluorescently labeled Tns proteins. All four required Tns proteins are present in the PTC in which we also find that the Tn7 ends are paired by protein-protein contacts between Tns proteins bound to the ends. Quantification of the relative amounts of the fluorescent Tns proteins in the PTC indicates that oligomers of TnsA, TnsB, and TnsC mediate Tn7 transposition. High-resolution DNA footprinting of the DNA product of transposition attTn7Colon, two colonsTn7 revealed that about 350 bp of DNA on the transposon ends and on attTn7 contact the Tns proteins. All seven binding sites for TnsB, the component of the transposase that specifically binds the ends and mediates 3' end breakage and joining, are occupied in the PTC. However, the protection pattern of the sites closest to the Tn7 ends in the PTC are different from that observed with TnsB alone, likely reflecting the pairing of the ends and their interaction with the target nucleoprotein complex necessary for activation of the breakage and joining steps. We also observe extensive protection of the attTn7 sequences in the PTC and that alternative DNA structures in substrate attTn7 that are imposed by TnsD are maintained in the PTC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.