ArticleBMC genomics2010
The ubiquitin-conjugating enzyme HR6B is required for maintenance of X chromosome silencing in mouse spermatocytes and spermatids.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 39 citations in OpenAlex.
- The role of ubiquitin-conjugating enzyme in the process of spermatogenesis.Reproductive biology and endocrinology : RB&E · 2024Review
- Article
- Destruction or Reconstruction: A Subtle Liaison between the Proteolytic and Signaling Role of Protein Ubiquitination in Spermatogenesis.Advances in experimental medicine and biology · 2021Article
- Article
- High-resolution transcriptional profiling of Anopheles gambiae spermatogenesis reveals mechanisms of sex chromosome regulation.Scientific reports · 2019Article
- Live cell analyses of synaptonemal complex dynamics and chromosome movements in cultured mouse testis tubules and embryonic ovaries.Chromosoma · 2018Article
- Article
- Non-Invasive Approaches to Epigenetic-Based Sperm Selection.Medical science monitor : international medical journal of experimental and clinical research · 2017Review
- Round Spermatid Injection Rescues Female Lethality of a Paternally Inherited Xist Deletion in Mouse.PLoS genetics · 2016Article
- Genomes of Ellobius species provide insight into the evolutionary dynamics of mammalian sex chromosomes.Genome research · 2016Article
- Expression and epigenomic landscape of the sex chromosomes in mouse post-meiotic male germ cells.Epigenetics & chromatin · 2016Article
- A functional variant in the UBE2B gene promoter is associated with idiopathic azoospermia.Reproductive biology and endocrinology : RB&E · 2015Article
- Incomplete meiotic sex chromosome inactivation in the domestic dog.BMC genomics · 2015Article
- Characterization of BRD4 during mammalian postmeiotic sperm development.Molecular and cellular biology · 2015Article
- Article
- UBE2B mRNA alterations are associated with severe oligozoospermia in infertile men.Molecular human reproduction · 2013Article
- Article
- RNF8 regulates active epigenetic modifications and escape gene activation from inactive sex chromosomes in post-meiotic spermatids.Genes & development · 2012Article
- Role of the ubiquitin-proteasome system in nervous system function and disease: using C. elegans as a dissecting tool.Cellular and molecular life sciences : CMLS · 2012Review
- Human male meiotic sex chromosome inactivation.PloS one · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe ubiquitin-conjugating enzyme HR6B is required for spermatogenesis in mouse. Loss of HR6B results in aberrant histone modification patterns on the trancriptionally silenced X and Y chromosomes (XY body) and on centromeric chromatin in meiotic prophase. We studied the relationship between these chromatin modifications and their effects on global gene expression patterns, in spermatocytes and spermatids.
resultsHR6B is enriched on the XY body and on centromeric regions in pachytene spermatocytes. Global gene expression analyses revealed that spermatid-specific single- and multicopy X-linked genes are prematurely expressed in Hr6b knockout spermatocytes. Very few other differences in gene expression were observed in these cells, except for upregulation of major satellite repeat transcription. In contrast, in Hr6b knockout spermatids, 7298 genes were differentially expressed; 65% of these genes was downregulated, but we observed a global upregulation of gene transcription from the X chromosome. In wild type spermatids, approximately 20% of the single-copy X-linked genes reach an average expression level that is similar to the average expression from autosomes.
conclusionsSpermatids maintain an enrichment of repressive chromatin marks on the X chromosome, originating from meiotic prophase, but this does not interfere with transcription of the single-copy X-linked genes that are reactivated or specifically activated in spermatids. HR6B represses major satellite repeat transcription in spermatocytes, and functions in the maintenance of X chromosome silencing in spermatocytes and spermatids. It is discussed that these functions involve modification of chromatin structure, possibly including H2B ubiquitylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.