ArticlePlant methods2010
UCE: A uracil excision (USER)-based toolbox for transformation of cereals.
Article in Plant methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- CRISPR/Cas9 and Transgene Verification of Gene Involvement in Unfolded Protein Response and Recombinant Protein Production in Barley Grain.Frontiers in plant science · 2021Article
- Transcriptional and Metabolic Changes Associated with Phytoglobin Expression during Germination of Barley Seeds.International journal of molecular sciences · 2020Article
- Modification of the Expression of the Aquaporin ZmPIP2;5 Affects Water Relations and Plant Growth.Plant physiology · 2020Article
- Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation.Journal of experimental botany · 2020Article
- Phytoglobin overexpression promotes barley growth in the presence of enhanced level of atmospheric nitric oxide.Journal of experimental botany · 2019Article
- Protein Targeting to Starch 1 is essential for starchy endosperm development in barley.Journal of experimental botany · 2019Article
- Reduced nitric oxide levels during drought stress promote drought tolerance in barley and is associated with elevated polyamine biosynthesis.Scientific reports · 2017Article
- Highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene.Scientific reports · 2017Article
- Barley HvPAPhy_a as transgene provides high and stable phytase activities in mature barley straw and in grains.Plant biotechnology journal · 2017Article
- Waxy and non-waxy barley cultivars exhibit differences in the targeting and catalytic activity of GBSS1a.Journal of experimental botany · 2017Article
- Barley plants over-expressing the NAC transcription factor gene HvNAC005 show stunting and delay in development combined with early senescence.Journal of experimental botany · 2016Article
- Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme.Nature communications · 2014Article
- Barley callus: a model system for bioengineering of starch in cereals.Plant methods · 2012Article
- Haemoglobin modulates salicylate and jasmonate/ethylene-mediated resistance mechanisms against pathogens.Journal of experimental botany · 2012Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCloning of gene casettes and other DNA sequences into the conventional vectors for biolistic or Agrobacterium-mediated transformation is hampered by a limited amount of unique restriction sites and by the difficulties often encountered when ligating small single strand DNA overhangs. These problems are obviated by "The Uracil Specific Excision Reagent (USER)" technology (New England Biolabs) which thus offers a new and very time-efficient method for engineering of big and complex plasmids.
resultsBy application of the USER system, we engineered a collection of binary vectors, termed UCE (USER cereal), ready for use in cloning of complex constructs into the T-DNA. A series of the vectors were tested and shown to perform successfully in Agrobacterium-mediated transformation of barley (Hordeum vulgare L.) as well as in biolistic transformation of endosperm cells conferring transient expression.
conclusionsThe USER technology is very well suited for generating a toolbox of vectors for transformation and it opens an opportunity to engineer complex vectors, where several genetic elements of different origin are combined in a single cloning reaction.
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