ArticleMarine biotechnology (New York, N.Y.)2011
Investigation on natural diets of larval marine animals using peptide nucleic acid-directed polymerase chain reaction clamping.
Article in Marine biotechnology (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 89 citations in OpenAlex.
- Contemporary eDNA methods complement conventional microscopy in zooplankton diet studies: Case study with American lobster postlarvae.PloS one · 2025Article
- Role of estuarine habitats for the feeding ecology of the European eel (Anguilla anguilla L.).PloS one · 2022Article
- Effectiveness of blocking primers and a peptide nucleic acid (PNA) clamp for 18S metabarcoding dietary analysis of herbivorous fish.PloS one · 2022Article
- A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus.BMC oral health · 2020Article
- Molecular diet analysis of Anguilliformes leptocephalus larvae collected in the western North Pacific.PloS one · 2019Article
- Host dietary specialization and neutral assembly shape gut bacterial communities of wild dragonflies.PeerJ · 2019Article
- Predatory flying squids are detritivores during their early planktonic life.Scientific reports · 2018Article
- Preliminary analysis of New Zealand scampi (PeerJ · 2018Article
- Molecular, Biochemical, and Dietary Regulation Features of α-Amylase in a Carnivorous Crustacean, the Spiny Lobster Panulirus argus.PloS one · 2016Article
- Trypsin isozymes in the lobster Panulirus argus (Latreille, 1804): from molecules to physiology.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2015Review
- Challenges to a molecular approach to prey identification in the Burmese python, Python molurus bivittatus.PeerJ · 2015Article
- Practical innovations for high-throughput amplicon sequencing.Nature methods · 2013Article
- Fussy feeders: phyllosoma larvae of the Western Rocklobster (Panulirus cygnus) demonstrate prey preference.PloS one · 2012Article
- Determining the diet of larvae of western rock lobster (Panulirus cygnus) using high-throughput DNA sequencing techniques.PloS one · 2012Article
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The stomach contents of the larvae of marine animals are usually very small in quantity and amorphous, especially in invertebrates, making morphological methods of identification very difficult. Nucleotide sequence analysis using polymerase chain reaction (PCR) is a likely approach, but the large quantity of larval (host) DNA present may mask subtle signals from the prey genome. We have adopted peptide nucleic acid (PNA)-directed PCR clamping to selectively inhibit amplification of host DNA for this purpose. The Japanese spiny lobster (Panulirus japonicus) and eel (Anguilla japonica) were used as model host and prey organisms, respectively. A lobster-specific PNA oligomer (20 bases) was designed to anneal to the sequence at the junction of the 18 S rDNA gene and the internal transcribed spacer 1 (ITS1) of the lobster. PCR using eukaryote universal primers for amplifying the ITS1 region used in conjunction with the lobster-specific PNA on a mixed DNA template of lobster and eel demonstrated successful inhibition of lobster ITS1 amplification while allowing efficient amplification of eel ITS1. This method was then applied to wild-caught lobster larvae of P. japonicus and P. longipes bispinosus collected around Ryukyu Archipelago, Japan. ITS1 sequences of a wide variety of animals (Ctenophora, Cnidaria, Crustacea, Teleostei, Mollusca, and Chaetognatha) were detected.
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