ArticleInternational journal of legal medicine2010
The cannabinoid receptor type 2 is time-dependently expressed during skeletal muscle wound healing in rats.
Article in International journal of legal medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Cannabinoid Receptors: An Update on Cell Signaling, Pathophysiological Roles and Therapeutic Opportunities in Neurological, Cardiovascular, and Inflammatory Diseases.International journal of molecular sciences · 2020Pooled it
- Endocannabinoid system and skeletal muscle health: Insights from cannabidiol.Molecular metabolism · 2026Review
- Cannabinoid type 2 receptor regulates skeletal muscle regeneration by NLRP3-GSDMD mediated macrophage pyroptosis after injury.Cell death discovery · 2026Article
- Detection of multiple biomarkers associated with satellite cell fate in the contused skeletal muscle of rats for wound age estimation.International journal of legal medicine · 2023Article
- Time-dependent expression of high-mobility group box-1 and toll-like receptors proteins as potential determinants of skin wound age in rats: Forensic implication.International journal of legal medicine · 2022Article
- Contusion concomitant with ischemia injury aggravates skeletal muscle necrosis and hinders muscle functional recovery.Experimental biology and medicine (Maywood, N.J.) · 2022Article
- The Endocannabinoid System: A Potential Target for the Treatment of Various Diseases.International journal of molecular sciences · 2021Review
- Vitality and wound-age estimation in forensic pathology: review and future prospects.Forensic sciences research · 2020Review
- Decisive role of P42/44 mitogen-activated protein kinase in ΔOncotarget · 2017Article
- Detection of RAGE expression and its application to diabetic wound age estimation.International journal of legal medicine · 2017Article
- Alternative Mechanism for White Adipose Tissue Lipolysis after Thermal Injury.Molecular medicine (Cambridge, Mass.) · 2016Article
- Detection of satellite cells during skeletal muscle wound healing in rats: time-dependent expressions of Pax7 and MyoD in relation to wound age.International journal of legal medicine · 2016Article
- α7nAChR is expressed in satellite cells at different myogenic status during skeletal muscle wound healing in rats.Journal of molecular histology · 2015Article
- Inhibition of FAAH confers increased stem cell migration via PPARα.Journal of lipid research · 2015Article
- CB2R orchestrates fibrogenesis through regulation of inflammatory response during the repair of skeletal muscle contusion.International journal of clinical and experimental pathology · 2015Article
- Selection of reference genes for expression analyses in liver of rats with impaired glucose metabolism.International journal of clinical and experimental pathology · 2015Article
- The time-dependent expression of α7nAChR during skeletal muscle wound healing in rats.International journal of legal medicine · 2014Article
- Time-dependent expression of SNAT2 mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation.Forensic science, medicine, and pathology · 2013Article
- Molecular pathology of brain edema after severe burns in forensic autopsy cases with special regard to the importance of reference gene selection.International journal of legal medicine · 2013Article
- Time-dependent expression and distribution of Egr-1 during skeletal muscle wound healing in rats.Journal of molecular histology · 2013Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the cannabinoid receptor type 2 (CB2R) was investigated by immunohistochemistry, Western blotting, and RT-PCR during wound healing of contused skeletal muscle in rats with attempt of its applicability to skeletal muscle wound age estimation. Furthermore, Macrophage Marker (MAC387) was utilized to identify macrophages recruited into injured skeletal muscle tissue. Co-localization of CB2R with Macrophage Marker was detected by confocal laser scanning microscopy. A total of 50 Sprague-Dawley male rats were divided into control and contusion groups (3 h, 6 h, 12 h, 1 day, 3 days, 5 days, 7 days, 10 days, and 14 days post-injury). In the uninjured controls, immunoreactivity of CB2R was detected in the sarcolemma and sarcoplasm of normal myofibers. In the contusion groups, a few polymorphonulcear cells, a large number of macrophages, and spindle-shaped fibroblastic cells showed a positive staining for CB2R in wounded zones. By Western blotting analysis, the average of CB2R to GAPDH ratios in 5-7 days post-injury groups was highest, and all the samples had ratios of >2.60. In the other groups, no samples showed ratios of >2.60 and the CB2R to GAPDH ratios ranged from 1.19 to 2.59. The expression tendency was also confirmed by RT-PCR. From the viewpoint of forensic pathology, these observations suggested that the ratio markedly exceeding 2.60 strongly indicated a wound age of 5-7 days. In conclusion, dynamic distribution and expression of CB2R suggest that CB2R be involved in modulating macrophages in response to inflammatory event in rat skeletal muscle wound healing and CB2R be available as a marker for wound age determination.
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