ArticlePlant cell reports2010
Genetic transformation of sweet sorghum.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Optimized Protocol for Sorghum Regeneration: Enhancing Embryogenic Callus Formation from Immature Inflorescences.Recent patents on biotechnology · 2026Article
- Association mapping and identification of candidate genes for callus induction and regeneration using sorghum mature seeds.Frontiers in plant science · 2025Article
- Sorghum as a monocot model for drought research.Frontiers in plant science · 2025Review
- Review
- Exploring Agrobacterium-mediated genetic transformation methods and its applications in Lilium.Plant methods · 2024Review
- Article
- New methods for sorghum transformation in temperate climates.AoB PLANTS · 2023Review
- Development of an in vitro regeneration system from immature inflorescences and CRISPR/Cas9-mediated gene editing in sudangrass.Journal, genetic engineering & biotechnology · 2023Article
- Morphogene-assisted transformation of Sorghum bicolor allows more efficient genome editing.Plant biotechnology journal · 2022Article
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy.Journal of experimental botany · 2022Review
- Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum.The New phytologist · 2021Article
- The Effect of Daminozide, Dark/Light Schedule and Copper Sulphate in Tissue Culture ofPlants (Basel, Switzerland) · 2021Article
- Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum.Biotechnology for biofuels · 2021Article
- Photosynthetic Regulation Under Salt Stress and Salt-Tolerance Mechanism of Sweet Sorghum.Frontiers in plant science · 2019Review
- Progress in Optimization ofInternational journal of molecular sciences · 2018Review
- Genetic engineering of grass cell wall polysaccharides for biorefining.Plant biotechnology journal · 2017Review
- Sweet sorghum as biofuel feedstock: recent advances and available resources.Biotechnology for biofuels · 2017Review
- Article
- Highly efficient sorghum transformation.Plant cell reports · 2012Article
- Embryogenic callus proliferation and regeneration conditions for genetic transformation of diverse sugarcane cultivars.Plant cell reports · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sweet sorghum has substantial potential as a biofuel feedstock, with advantages in some environments over alternatives such as sugarcane or maize. Gene technologies are likely to be important to achieve yields sufficient for food, fuel and fibre production from available global croplands, but sorghum has proven difficult to transform. Tissue culture recalcitrance and poor reproducibility of transformation protocols remain major challenges for grain sorghum, and there has been no reported success for sweet sorghum. Here we describe a repeatable transformation system for sweet sorghum, based on (1) optimized tissue culture conditions for embryogenic callus production with >90% regenerability in 12-week-old calli, and (2) an effective selection regimen for hygromycin resistance conferred by a Ubi-hpt transgene following particle bombardment. Using this method, we have produced sixteen independent transgenic lines from multiple batches at an overall efficiency of 0.09% transformants per excised immature embryo. Co-expression frequency of a non-selected luciferase reporter was 62.5%. Transgene integration and expression were confirmed in T(0) and T(1) plants by Southern analysis and luciferase assays. This success using the major international sweet sorghum cultivar Ramada provides a foundation for molecular improvement of sweet sorghum through the use of transgenes. Factors likely to be important for success with other sweet sorghum cultivars are identified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.