ArticleIndian journal of ophthalmology
Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
Article in Indian journal of ophthalmology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Renaissance of an Emerging Pathogen-Clinical ophthalmology (Auckland, N.Z.) · 2025Article
- From Clinical Suspicion to Diagnosis: A Review of Diagnostic Approaches and Challenges in Fungal Keratitis.Journal of clinical medicine · 2024Review
- Review
- Diagnostic methods for the etiological assessment of infectious corneal pathology (Review).Experimental and therapeutic medicine · 2022Review
- Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture Outcome.Journal of clinical medicine · 2021Article
- A deep learning approach in diagnosing fungal keratitis based on corneal photographs.Scientific reports · 2020Article
- Comparative Analysis of Clinical and Environmental Strains ofFrontiers in microbiology · 2020Article
- Does the sampling instrument influence corneal culture outcome in patients with infectious keratitis? A retrospective study comparing cotton tipped applicator with knife blade.BMJ open ophthalmology · 2020Article
- An Omics Approach to Diagnosing or Investigating Fungal Keratitis.International journal of molecular sciences · 2019Review
- Characterization of a Halotolerant Fungus from a Marine Sponge.BioMed research international · 2019Article
- Cell Wall Synthesis, Development of Hyphae and Metabolic Pathways Are Processes Potentially Regulated by MicroRNAs Produced Between the Morphological Stages ofFrontiers in microbiology · 2018Article
- Staining with methylthioninium chloride for the diagnosis of fungal keratitis.Experimental and therapeutic medicine · 2013Article
- Is blood agar an alternative to sabouraud dextrose agar for the isolation of fungi in patients with mycotic keratitis.International ophthalmology · 2013Article
- Diagnosis of infectious diseases of the eye.Eye (London, England) · 2012Review
- In silico characterization of microRNAs-like sequences in the genome of Paracoccidioides brasiliensis.Genetics and molecular biologyArticle
- Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?Indian journal of ophthalmologyArticle
- Comment on: is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?Indian journal of ophthalmologyArticle
- Sabouraud dextrose agar for the diagnosis of fungal keratitis.Indian journal of ophthalmologyArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. MATERIALS AND
methodsCorneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with Escherichia coli overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification.
resultsTwenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, P = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in in vitro experiment although the characteristic spores and color were seen earlier on SDA.
conclusionFungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus.
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