ArticleMolecular biology of the cell2010
Emi2-mediated inhibition of E2-substrate ubiquitin transfer by the anaphase-promoting complex/cyclosome through a D-box-independent mechanism.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- MOS is a key regulator of meiotic midbody assembly and prevents abnormal divisions in mouse eggs and their polar bodies.Journal of cell science · 2026Article
- YY1 activates EMI2 and promotes the progression of cholangiocarcinoma through the PI3K/Akt signaling axis.Cancer cell international · 2021Article
- Calcineurin promotes APC/C activation at meiotic exit by acting on both XErp1 and Cdc20.EMBO reports · 2018Article
- The zinc-binding region (ZBR) fragment of Emi2 can inhibit APC/C by targeting its association with the coactivator Cdc20 and UBE2C-mediated ubiquitylation.FEBS open bio · 2014Article
- Modulation of cell cycle control during oocyte-to-embryo transitions.The EMBO journal · 2013Review
- Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex.Nature cell biology · 2013Article
- EMI1, a three-in-one ubiquitylation inhibitor.Nature structural & molecular biology · 2013Article
- Electron microscopy structure of human APC/C(CDH1)-EMI1 reveals multimodal mechanism of E3 ligase shutdown.Nature structural & molecular biology · 2013Article
- The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1.The Journal of cell biology · 2012Article
- Cubism and the cell cycle: the many faces of the APC/C.Nature reviews. Molecular cell biology · 2011Review
- State of the APC/C: organization, function, and structure.Critical reviews in biochemistry and molecular biology · 2011Review
- Mouse Emi2 as a distinctive regulatory hub in second meiotic metaphase.Development (Cambridge, England) · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Vertebrate eggs are arrested at Metaphase II by Emi2, the meiotic anaphase-promoting complex/cyclosome (APC/C) inhibitor. Although the importance of Emi2 during oocyte maturation has been widely recognized and its regulation extensively studied, its mechanism of action remained elusive. Many APC/C inhibitors have been reported to act as pseudosubstrates, inhibiting the APC/C by preventing substrate binding. Here we show that a previously identified zinc-binding region is critical for the function of Emi2, whereas the D-box is largely dispensable. We further demonstrate that instead of acting through a "pseudosubstrate" mechanism as previously hypothesized, Emi2 can inhibit Cdc20-dependent activation of the APC/C substoichiometrically, blocking ubiquitin transfer from the ubiquitin-charged E2 to the substrate. These findings provide a novel mechanism of APC/C inhibition wherein the final step of ubiquitin transfer is targeted and raise the interesting possibility that APC/C is inhibited by Emi2 in a catalytic manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.