ArticleProceedings of the National Academy of Sciences of the United States of America2010
Single cell trapping and DNA damage analysis using microwell arrays.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
111 citing papers in PubMed, 259 citations in OpenAlex.
- Association of mitochondrial DNA haplogroup, oxidative DNA damage and inflammatory markers in middle-aged adults.International journal of radiation biology · 2026Article
- Curcumin Attenuates Aroclor 1254-Induced Oxidative, Genotoxic, and Apoptotic Alterations in HepG2 Cells.Journal of biochemical and molecular toxicology · 2026Article
- New Approach Methods in Genetic Toxicology.Environmental and molecular mutagenesis · 2026Article
- The Comet Assay in Human Biomonitoring.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Single-Cell Nucleus Extraction with Cellular Indexing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Utilization of the CometChip assay for detecting PAH-induced DNA bulky adducts in a 3D primary human bronchial epithelial cell model.Toxicology · 2025Article
- Transferability and Reproducibility of the HepaRG CometChip Assay.Environmental and molecular mutagenesis · 2025Article
- Paired Analyses of Nuclear Protein Targets and Genomic DNA by Single-Cell Western Blot and Single-Cell PCR.Analytical chemistry · 2025Article
- Article
- Digital PCR for Single-Cell Analysis.Biosensors · 2024Review
- Double Digital Assay for Single Extracellular Vesicle and Single Molecule Detection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Chemical Insights into Oxidative and Nitrative Modifications of DNA.International journal of molecular sciences · 2023Review
- Frailty, sex, and poverty are associated with DNA damage and repair in frail, middle-aged urban adults.DNA repair · 2023Article
- Sonic snares: Trapping microorganisms for deeper insights into swimming behavior and ciliary dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Development of a Novel, Automated High-Throughput Device for Performing the Comet Assay.International journal of molecular sciences · 2023Article
- Skin Immuno-CometChip in 3D vs. 2D Cultures to Screen Topical Toxins and Skin-Specific Cytochrome Inducers.Genes · 2023Article
- Measuring DNA modifications with the comet assay: a compendium of protocols.Nature protocols · 2023Review
- Advances in Microfluidics for Single Red Blood Cell Analysis.Biosensors · 2023Review
- Novel In Vivo CometChip Reveals NDMA-Induced DNA Damage and Repair in Multiple Mouse Tissues.International journal of molecular sciences · 2022Article
- Using the HepaCometChip Assay for Broad-Spectrum DNA Damage Analysis.Current protocols · 2022Article
51 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
With a direct link to cancer, aging, and heritable diseases as well as a critical role in cancer treatment, the importance of DNA damage is well-established. The intense interest in DNA damage in applications ranging from epidemiology to drug development drives an urgent need for robust, high throughput, and inexpensive tools for objective, quantitative DNA damage analysis. We have developed a simple method for high throughput DNA damage measurements that provides information on multiple lesions and pathways. Our method utilizes single cells captured by gravity into a microwell array with DNA damage revealed morphologically by gel electrophoresis. Spatial encoding enables simultaneous assays of multiple experimental conditions performed in parallel with fully automated analysis. This method also enables novel functionalities, including multiplexed labeling for parallel single cell assays, as well as DNA damage measurement in cell aggregates. We have also developed 24- and 96-well versions, which are applicable to high throughput screening. Using this platform, we have quantified DNA repair capacities of individuals with different genetic backgrounds, and compared the efficacy of potential cancer chemotherapeutics as inhibitors of a critical DNA repair enzyme, human AP endonuclease. This platform enables high throughput assessment of multiple DNA repair pathways and subpathways in parallel, thus enabling new strategies for drug discovery, genotoxicity testing, and environmental health.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.